RT Journal Article SR Electronic(1) A1 Wressnigg, Nina A1 Shurygina, Anna Polina A1 Wolff, Thorsten A1 Redlberger-Fritz, Monika A1 Popow-Kraupp, Therese A1 Muster, Thomas A1 Egorov, Andrej A1 Kittel, ChristianYR 2009 T1 Influenza B mutant viruses with truncated NS1 proteins grow efficiently in Vero cells and are immunogenic in mice JF Journal of General Virology, VO 90 IS 2 SP 366 OP 374 DO https://doi.org/10.1099/vir.0.006122-0 PB Microbiology Society, SN 1465-2099, AB Contemporary influenza B virus strains were generated encoding C-terminally truncated NS1 proteins. Viable viruses containing the N-terminal 14, 38, 57 or 80 aa of the NS1 protein were rescued in Vero cells. The influenza B virus NS1-truncated mutants were impaired in their ability to counteract interferon (IFN) production, induce antiviral pro-inflammatory cytokines early after infection and show attenuated or restricted growth in IFN-competent hosts. In Vero cells, all of the mutant viruses replicated to high titres comparable to the wild-type influenza B virus. Mice that received a single, intranasal immunization of the NS1-truncated mutants elicited an antibody response and protection against wild-type virus challenge. Therefore, these NS1-truncated mutants should prove useful as potential candidates for live-attenuated influenza virus vaccines., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.006122-0