@article{mbs:/content/journal/jgv/10.1099/vir.0.010660-0, author = "Varich, Natalia L. and Kochergin-Nikitsky, Konstantin S. and Usachev, Evgeny V. and Usacheva, Olga V. and Prilipov, Alexei G. and Webster, Robert G. and Kaverin, Nikolai V.", title = "Location of antigenic sites recognized by monoclonal antibodies in the influenza A virus nucleoprotein molecule", journal= "Journal of General Virology", year = "2009", volume = "90", number = "7", pages = "1730-1733", doi = "https://doi.org/10.1099/vir.0.010660-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.010660-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The locations of amino acid positions relevant to antigenic variation in the nucleoprotein (NP) of influenza virus are not conclusively known. We analysed the antigenic structure of influenza A virus NP by introducing site-specific mutations at amino acid positions presumed to be relevant for the differentiation of strain differences by anti-NP monoclonal antibodies. Mutant proteins were expressed in a prokaryotic system and analysed by performing ELISA with monoclonal antibodies. Four amino acid residues were found to determine four different antibody-binding sites. When mapped in a 3D X-ray model of NP, the four antigenically relevant amino acid positions were found to be located in separate physical sites of the NP molecule.", }