Location of antigenic sites recognized by monoclonal antibodies in the influenza A virus nucleoprotein molecule
Varich, Natalia L. and Kochergin-Nikitsky, Konstantin S. and Usachev, Evgeny V. and Usacheva, Olga V. and Prilipov, Alexei G. and Webster, Robert G. and Kaverin, Nikolai V.,, 90, 1730-1733 (2009),
doi = https://doi.org/10.1099/vir.0.010660-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= The locations of amino acid positions relevant to antigenic variation in the nucleoprotein (NP) of influenza virus are not conclusively known. We analysed the antigenic structure of influenza A virus NP by introducing site-specific mutations at amino acid positions presumed to be relevant for the differentiation of strain differences by anti-NP monoclonal antibodies. Mutant proteins were expressed in a prokaryotic system and analysed by performing ELISA with monoclonal antibodies. Four amino acid residues were found to determine four different antibody-binding sites. When mapped in a 3D X-ray model of NP, the four antigenically relevant amino acid positions were found to be located in separate physical sites of the NP molecule.,
language=,
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