@article{mbs:/content/journal/jgv/10.1099/vir.0.012815-0, author = "Tsao, Edward H. and Kellam, Paul and Sin, Cheryl S. Y. and Rasaiyaah, Jane and Griffiths, Paul D. and Clark, Duncan A.", title = "Microarray-based determination of the lytic cascade of human herpesvirus 6B", journal= "Journal of General Virology", year = "2009", volume = "90", number = "11", pages = "2581-2591", doi = "https://doi.org/10.1099/vir.0.012815-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.012815-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The lytic gene expression of several members of the human herpesvirus family has been profiled by using gene-expression microarrays; however, the lytic cascade of roseoloviruses has not been studied in similar depth. Based on the complete DNA genome sequences of human herpesvirus 6 variant A (HHV-6A) and variant B (HHV-6B), we constructed a cDNA microarray containing DNA probes to their predicted open reading frames, plus 914 human genes. Gene-expression profiling of HHV-6B strain Z29 in SupT1 cells over a 60 h time-course post-infection, together with kinetic classification of the HHV-6B genes in the presence of either cycloheximide or phosphonoacetic acid, allowed the placement of HHV-6B genes into defined kinetic classes. Eighty-nine HHV-6B genes were divided into four different expression kinetic classes: eight immediate-early, 44 early, 33 late and four biphasic. Clustering of genes with similar expression profiles implied a shared function, thus revealing possible roles of previously uncharacterized HHV-6B genes.", }