%0 Journal Article %A Tsao, Edward H. %A Kellam, Paul %A Sin, Cheryl S. Y. %A Rasaiyaah, Jane %A Griffiths, Paul D. %A Clark, Duncan A. %T Microarray-based determination of the lytic cascade of human herpesvirus 6B %D 2009 %J Journal of General Virology, %V 90 %N 11 %P 2581-2591 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.012815-0 %I Microbiology Society, %X The lytic gene expression of several members of the human herpesvirus family has been profiled by using gene-expression microarrays; however, the lytic cascade of roseoloviruses has not been studied in similar depth. Based on the complete DNA genome sequences of human herpesvirus 6 variant A (HHV-6A) and variant B (HHV-6B), we constructed a cDNA microarray containing DNA probes to their predicted open reading frames, plus 914 human genes. Gene-expression profiling of HHV-6B strain Z29 in SupT1 cells over a 60 h time-course post-infection, together with kinetic classification of the HHV-6B genes in the presence of either cycloheximide or phosphonoacetic acid, allowed the placement of HHV-6B genes into defined kinetic classes. Eighty-nine HHV-6B genes were divided into four different expression kinetic classes: eight immediate-early, 44 early, 33 late and four biphasic. Clustering of genes with similar expression profiles implied a shared function, thus revealing possible roles of previously uncharacterized HHV-6B genes. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.012815-0