RT Journal Article SR Electronic(1) A1 Tsao, Edward H. A1 Kellam, Paul A1 Sin, Cheryl S. Y. A1 Rasaiyaah, Jane A1 Griffiths, Paul D. A1 Clark, Duncan A.YR 2009 T1 Microarray-based determination of the lytic cascade of human herpesvirus 6B JF Journal of General Virology, VO 90 IS 11 SP 2581 OP 2591 DO https://doi.org/10.1099/vir.0.012815-0 PB Microbiology Society, SN 1465-2099, AB The lytic gene expression of several members of the human herpesvirus family has been profiled by using gene-expression microarrays; however, the lytic cascade of roseoloviruses has not been studied in similar depth. Based on the complete DNA genome sequences of human herpesvirus 6 variant A (HHV-6A) and variant B (HHV-6B), we constructed a cDNA microarray containing DNA probes to their predicted open reading frames, plus 914 human genes. Gene-expression profiling of HHV-6B strain Z29 in SupT1 cells over a 60 h time-course post-infection, together with kinetic classification of the HHV-6B genes in the presence of either cycloheximide or phosphonoacetic acid, allowed the placement of HHV-6B genes into defined kinetic classes. Eighty-nine HHV-6B genes were divided into four different expression kinetic classes: eight immediate-early, 44 early, 33 late and four biphasic. Clustering of genes with similar expression profiles implied a shared function, thus revealing possible roles of previously uncharacterized HHV-6B genes., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.012815-0