1887

Abstract

Human bocavirus (HBoV) was recently discovered in children with respiratory-tract infection and has been detected frequently in faecal specimens from children with gastroenteritis. The present study addresses for the first time, to our knowledge, the prevalence of HBoV in river water. By using a newly developed real-time PCR targeting a conserved region of the NP1 gene of HBoV, virus levels in water samples were determined. Moreover, partial sequence analysis of the NP1 gene of HBoV and comparative phylogenetic analysis were performed. HBoV was detected in 40.8 % of collected water samples. The virus level ranged between 3×10 and 2×10 genome equivalents l. Therefore, the present study suggests that river water could play a role in the spread of HBoV. However, further work should be done to determine the actual risk of infection via surface water.

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2009-11-01
2024-04-20
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References

  1. Allander T., Tammi M. T., Eriksson M., Bjerkner A., Tiveljung-Lindell A., Andersson B. 2005; Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proc Natl Acad Sci U S A 102:12891–12896 [CrossRef]
    [Google Scholar]
  2. Campe H., Hartberger C., Sing A. 2008; Role of human bocavirus infections in outbreaks of gastroenteritis. J Clin Virol 43:340–342 [CrossRef]
    [Google Scholar]
  3. Chow B. D., Huang Y. T., Esper F. P. 2008; Evidence of human bocavirus circulating in children and adults, Cleveland, Ohio. J Clin Virol 43:302–306 [CrossRef]
    [Google Scholar]
  4. Durham P. J., Lax A., Johnson R. H. 1985; Pathological and virological studies of experimental parvoviral enteritis in calves. Res Vet Sci 38:209–219
    [Google Scholar]
  5. Hamza I. A., Jurzik L., Stang A., Sure K., Uberla K., Wilhelm M. 2009; Detection of human viruses in rivers of a densly-populated area in Germany using a virus adsorption elution method optimized for PCR analyses. Water Res 43:2657–2668 [CrossRef]
    [Google Scholar]
  6. Haramoto E., Katayama H., Oguma K., Ohgaki S. 2007; Recovery of naked viral genomes in water by virus concentration methods. J Virol Methods 142:169–173 [CrossRef]
    [Google Scholar]
  7. Klempa B., Kruger D. H., Auste B., Stanko M., Krawczyk A., Nickel K. F., Uberla K., Stang A. 2009; A novel cardiotropic murine adenovirus representing a distinct species of mastadenoviruses. J Virol 83:5749–5759 [CrossRef]
    [Google Scholar]
  8. Kukkula M., Arstila P., Klossner M. L., Maunula L., Bonsdorff C. H., Jaatinen P. 1997; Waterborne outbreak of viral gastroenteritis. Scand J Infect Dis 29:415–418 [CrossRef]
    [Google Scholar]
  9. Kupfer B., Vehreschild J., Cornely O., Kaiser R., Plum G., Viazov S., Franzen C., Tillmann R. L., Simon A. other authors 2006; Severe pneumonia and human bocavirus in adult. Emerg Infect Dis 12:1614–1616 [CrossRef]
    [Google Scholar]
  10. Lee S. H., Kim S. J. 2002; Detection of infectious enteroviruses and adenoviruses in tap water in urban areas in Korea. Water Res 36:248–256 [CrossRef]
    [Google Scholar]
  11. Lee J. I., Chung J. Y., Han T. H., Song M. O., Hwang E. S. 2007; Detection of human bocavirus in children hospitalized because of acute gastroenteritis. J Infect Dis 196:994–997 [CrossRef]
    [Google Scholar]
  12. Lodder W. J., de Roda Husman A. M. 2005; Presence of noroviruses and other enteric viruses in sewage and surface waters in the Netherlands. Appl Environ Microbiol 71:1453–1461 [CrossRef]
    [Google Scholar]
  13. Nuanualsuwan S., Cliver D. O. 2002; Pretreatment to avoid positive RT-PCR results with inactivated viruses. J Virol Methods 104:217–225 [CrossRef]
    [Google Scholar]
  14. Pina S., Puig M., Lucena F., Jofre J., Girones R. 1998; Viral pollution in the environment and in shellfish: human adenovirus detection by PCR as an index of human viruses. Appl Environ Microbiol 64:3376–3382
    [Google Scholar]
  15. Pozo F., Garcia-Garcia M. L., Calvo C., Cuesta I., Perez-Brena P., Casas I. 2007; High incidence of human bocavirus infection in children in Spain. J Clin Virol 40:224–228 [CrossRef]
    [Google Scholar]
  16. Rozen S., Skaletsky H. J. 2000; Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics Methods and Protocols: Methods in Molecular Biology pp 365–386Edited by Krawetz S., Misener S. Totowa, NJ: Humana Press;
    [Google Scholar]
  17. Srivastava R. N., Lund E. 1980; The stability of bovine parvovirus and its possible use as an indicator for the persistence of enteric viruses. Water Res 14:1017–1021 [CrossRef]
    [Google Scholar]
  18. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  19. Tozer S. J., Lambert S. B., Whiley D. M., Bialasiewicz S., Lyon M. J., Nissen M. D., Sloots T. P. 2009; Detection of human bocavirus in respiratory, fecal, and blood samples by real-time PCR. J Med Virol 81:488–493 [CrossRef]
    [Google Scholar]
  20. van Heerden J., Ehlers M. M., Heim A., Grabow W. O. K. 2005; Prevalence, quantification and typing of adenoviruses detected in river and treated drinking water in South Africa. J Appl Microbiol 99:234–242 [CrossRef]
    [Google Scholar]
  21. Vicente D., Cilla G., Montes M., Perez-Yarza E. G., Perez-Trallero E. 2007; Human bocavirus, a respiratory and enteric virus. Emerg Infect Dis 13:636–637 [CrossRef]
    [Google Scholar]
  22. Weissbrich B., Neske F., Schubert J., Tollmann F., Blath K., Blessing K., Kreth H. W. 2006; Frequent detection of bocavirus DNA in German children with respiratory tract infections. BMC Infect Dis 6:109 [CrossRef]
    [Google Scholar]
  23. Ziegler S., Tillmann R. L., Müller A., Simon A., Schildgen V., Schildgen O. 2008; No gastroenteric bocavirus in high risk patients stool samples. J Clin Virol 43:349–350 [CrossRef]
    [Google Scholar]
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