1887

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Volume 91, Issue 5

Analysis of cassava brown streak viruses reveals the presence of distinct virus species causing cassava brown streak disease in East Africa Free

Abstract

Cassava brown streak virus (CBSV) isolates were analysed from symptomatic cassava collected between 1997 and 2008 in the major cultivation regions of East Africa. An analysis of complete RNA genomes of seven isolates from Kenya, Tanzania, Mozambique, Uganda and Malawi revealed a common genome structure, but the isolates clearly clustered in two distinct clades. The first comprised isolates from Kenya, Uganda, Malawi, north-western Tanzania and the CBSV described previously, and shared between 87 and 95 % nucleotide sequence identity, whilst the second included isolates from coastal regions of Mozambique and Tanzania, which shared only 70 % nucleotide sequence identities with isolates of the first clade. When the amino acid sequences of viral proteins were compared, identities as low as 47 % (Ham1) and 59 % (P1) between the two clades were found. An antiserum obtained against the capsid protein of a clade 1 isolate identified a 43 kDa protein in clade 1 isolates and a 45 kDa protein in clade 2 isolates. Several cassava cultivars were susceptible to isolates of clade 2 but resistant to those of clade 1. The differences observed both in biological behaviour and in genomic and protein sequences indicate that cassava brown streak disease in East Africa is caused by at least two distinct virus species. It is suggested that those of clade 1 retain the species name , whilst those of clade 2 be classified as .

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2010-05-01
2024-04-18
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References

  1. Adams, M. J., Antoniw, J. F. & Beaudoin, F.(2005a). Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Mol Plant Pathol 6, 471–487.[CrossRef] [Google Scholar]
  2. Adams, M. J., Antoniw, J. F. & Fauquet, C. M.(2005b). Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol 150, 459–479.[CrossRef] [Google Scholar]
  3. Alicai, T., Omongo, C. A., Maruthi, M. N., Hillocks, R. J., Baguma, Y., Kawuki, R., Bua, A., Otim-Nape, G. W. & Colvin, J.(2007). Re-emergence of cassava brown streak disease in Uganda. Plant Dis 91, 24–29.[CrossRef] [Google Scholar]
  4. Dizadji, A., Koohi-Habibi, M., Izadpanah, K., Dietrich, C., Mossahebi, G. H. & Winter, S.(2008). Characterisation of lettuce virus X, a new potexvirus infecting lettuce in Iran. Arch Virol 153, 1867–1875.[CrossRef] [Google Scholar]
  5. Fauquet, C. M., Mayo, M. A., Maniloff, J., Desselberger, U. & Ball, L. A. (eds)(2005).Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses. San Diego: Elsevier.
  6. Harrison, B. D., Lennon, A. M. & Aiton, M. M.(1986). Properties of four previously uncharacterized cassava viruses. Phytopathology 76, 1075 [Google Scholar]
  7. Hillocks, R. J. & Jennings, D. L.(2003). Cassava brown streak disease: a review of present knowledge and research needs. Int J Pest Manage 49, 225–234.[CrossRef] [Google Scholar]
  8. Janssen, D., Martín, G., Velasco, L., Gómez, P., Segundo, E., Ruiz, L. & Cuadrado, I. M.(2005). Absence of a coding region for the helper component-proteinase in the genome of cucumber vein yellowing virus, a whitefly-transmitted member of the Potyviridae. Arch Virol 150, 1439–1447.[CrossRef] [Google Scholar]
  9. Li, W., Hilf, M. E., Webb, S. E., Baker, C. A. & Adkins, S.(2008). Presence of P1b and absence of HC-Pro in Squash vein yellowing virus suggests a general feature of the genus Ipomovirus in the family Potyviridae. Virus Res 135, 213–219.[CrossRef] [Google Scholar]
  10. Lister, R. M.(1959). Mechanical transmission of cassava brown streak virus. Nature 183, 1588–1589. [Google Scholar]
  11. Mbanzibwa, D. R., Tian, Y., Mukasa, S. B. & Valkonen, J. P.(2009a). Cassava brown streak virus (Potyviridae) encodes a putative Maf/HAM1 pyrophosphatase implicated in reduction of mutations and a P1 proteinase that suppresses RNA silencing but contains no HC-Pro. J Virol 83, 6934–6940.[CrossRef] [Google Scholar]
  12. Mbanzibwa, D. R., Tian, Y. P., Tugume, A. K., Mukasa, S. B., Tairo, F., Kyamanywa, S., Kullaya, A. & Valkonen, J. P. T.(2009b). Genetically distinct strains of Cassava brown streak virus in the Lake Victoria basin and the Indian Ocean coastal area of East Africa. Arch Virol 154, 353–359.[CrossRef] [Google Scholar]
  13. Monger, W. A., Seal, S., Cotton, S. & Foster, G. D.(2001a). Identification of different isolates of Cassava brown streak virus and development of a diagnostic test. Plant Pathol 50, 768–775.[CrossRef] [Google Scholar]
  14. Monger, W. A., Seal, S., Isaac, A. M. & Foster, G. D.(2001b). Molecular characterization of the Cassava brown streak virus coat protein. Plant Pathol 50, 527–534.[CrossRef] [Google Scholar]
  15. Nichols, R. F. W.(1950). The brown streak disease of cassava: distribution climatic effects and diagnostic symptoms. East Afr Agric J 15, 154–160. [Google Scholar]
  16. Polidoros, A. N., Pasentsis, K. & Tsaftaris, A. S.(2006). Rolling circle amplification-RACE: a method for simultaneous isolation of 5′ and 3′ cDNA ends from amplified cDNA templates. Biotechniques 41, 35[CrossRef] [Google Scholar]
  17. Raji, A., Ladeinde, O. & Dixon, A.(2008). Screening landraces for additional sources of field resistance to cassava mosaic disease and green mite for integration into the cassava improvement program. J Integr Plant Biol 50, 311–318.[CrossRef] [Google Scholar]
  18. Rzhetsky, A. & Nei, M.(1992). A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9, 945–967. [Google Scholar]
  19. Storey, H. H.(1936). Virus diseases of East African plants. VI. A progress report on studies of the diseases of cassava. East Afr Agric J 2, 34–39. [Google Scholar]
  20. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  21. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  22. Valli, A., López-Moya, J. J. & García, J. A.(2007). Recombination and gene duplication in the evolutionary diversification of P1 proteins in the family Potyviridae. J Gen Virol 88, 1016–1028.[CrossRef] [Google Scholar]
  23. Were, H. K., Winter, S. & Maiss, E.(2004a). Occurrence and distribution of cassava begomoviruses in Kenya. Ann Appl Biol 145, 175–184.[CrossRef] [Google Scholar]
  24. Were, H. K., Winter, S. & Maiss, E.(2004b). Viruses infecting cassava in Kenya. Plant Dis 88, 17–22.[CrossRef] [Google Scholar]
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Analysis of cassava brown streak viruses reveals the presence of distinct virus species causing cassava brown streak disease in East Africa
J. Gen. Virol. 91, 1365 (2010); https://doi.org/10.1099/vir.0.014688-0
/content/journal/jgv/10.1099/vir.0.014688-0
/content/journal/jgv/10.1099/vir.0.014688-0
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