@article{mbs:/content/journal/jgv/10.1099/vir.0.014852-0, author = "Tuke, Philip W. and Parry, Ruth P. and Appleton, Hazel", title = "Parvovirus PARV4 visualization and detection", journal= "Journal of General Virology", year = "2010", volume = "91", number = "2", pages = "541-544", doi = "https://doi.org/10.1099/vir.0.014852-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.014852-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The parvovirus PARV4 is the most recently described member of the family Parvoviridae that has a human host. To investigate the prevalence of PARV4 in blood, a quantitative TaqMan PCR was developed and plasma, sera or whole blood from a variety of population groups were examined. Eight samples were positive for PARV4, one at high copy number. The high-titre-positive plasma had an approximate viral load of 5×108 genome equivalents ml−1. Two human sera, identified as PARV4 antibody-positive by indirect immunofluorescence, were used in immune electron microscopy to try to visualize native PARV4 within the high-titre human plasma. PARV4 particles were observed using one of these two sera. To our knowledge, this is the first time that native PARV4 has been visualized.", }