RT Journal Article SR Electronic(1) A1 Tuke, Philip W. A1 Parry, Ruth P. A1 Appleton, HazelYR 2010 T1 Parvovirus PARV4 visualization and detection JF Journal of General Virology, VO 91 IS 2 SP 541 OP 544 DO https://doi.org/10.1099/vir.0.014852-0 PB Microbiology Society, SN 1465-2099, AB The parvovirus PARV4 is the most recently described member of the family Parvoviridae that has a human host. To investigate the prevalence of PARV4 in blood, a quantitative TaqMan PCR was developed and plasma, sera or whole blood from a variety of population groups were examined. Eight samples were positive for PARV4, one at high copy number. The high-titre-positive plasma had an approximate viral load of 5×108 genome equivalents ml−1. Two human sera, identified as PARV4 antibody-positive by indirect immunofluorescence, were used in immune electron microscopy to try to visualize native PARV4 within the high-titre human plasma. PARV4 particles were observed using one of these two sera. To our knowledge, this is the first time that native PARV4 has been visualized., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.014852-0