%0 Journal Article %A Michel, B. %A Leroy, B. %A Stalin Raj, V. %A Lieffrig, F. %A Mast, J. %A Wattiez, R. %A Vanderplasschen, A. F. %A Costes, B. %T The genome of cyprinid herpesvirus 3 encodes 40 proteins incorporated in mature virions %D 2010 %J Journal of General Virology, %V 91 %N 2 %P 452-462 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.015198-0 %I Microbiology Society, %X Koi herpesvirus, also known as cyprinid herpesvirus 3 (CyHV-3), is the aetiological agent of an emerging and mortal disease in common and koi carp. CyHV-3 virions present the characteristic morphology of other members of the order Herpesvirales, being composed of an envelope, a capsid containing the genome and a tegument. This study identified CyHV-3 structural proteins and the corresponding encoding genes using liquid chromatography tandem mass spectrometry-based proteomic approaches. In addition, exponentially modified protein abundance index analyses were used to estimate the relative abundance of the identified proteins in CyHV-3 virions. These analyses resulted in the identification of 40 structural proteins, which were classified based on bioinformatic analyses as capsid (three), envelope (13), tegument (two) and unclassified (22) structural proteins. Finally, a search for host proteins in purified CyHV-3 virions indicated the potential incorporation of up to 18 distinct cellular proteins. The identification of the proteins incorporated into CyHV-3 virions and determination of the viral genes encoding these proteins are key milestones for further fundamental and applied research on this virus. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.015198-0