1887

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Volume 91, Issue 2

The genome of cyprinid herpesvirus 3 encodes 40 proteins incorporated in mature virions Free

Abstract

Koi herpesvirus, also known as cyprinid herpesvirus 3 (CyHV-3), is the aetiological agent of an emerging and mortal disease in common and koi carp. CyHV-3 virions present the characteristic morphology of other members of the order , being composed of an envelope, a capsid containing the genome and a tegument. This study identified CyHV-3 structural proteins and the corresponding encoding genes using liquid chromatography tandem mass spectrometry-based proteomic approaches. In addition, exponentially modified protein abundance index analyses were used to estimate the relative abundance of the identified proteins in CyHV-3 virions. These analyses resulted in the identification of 40 structural proteins, which were classified based on bioinformatic analyses as capsid (three), envelope (13), tegument (two) and unclassified (22) structural proteins. Finally, a search for host proteins in purified CyHV-3 virions indicated the potential incorporation of up to 18 distinct cellular proteins. The identification of the proteins incorporated into CyHV-3 virions and determination of the viral genes encoding these proteins are key milestones for further fundamental and applied research on this virus.

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2010-02-01
2024-04-20
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The genome of cyprinid herpesvirus 3 encodes 40 proteins incorporated in mature virions
J. Gen. Virol. 91, 452 (2010); https://doi.org/10.1099/vir.0.015198-0
/content/journal/jgv/10.1099/vir.0.015198-0
/content/journal/jgv/10.1099/vir.0.015198-0
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