@article{mbs:/content/journal/jgv/10.1099/vir.0.015396-0, author = "Herd, Karen A. and Nelson, Michelle and Mahalingam, Suresh and Tindle, Robert W.", title = "Pulmonary infection of mice with human metapneumovirus induces local cytotoxic T-cell and immunoregulatory cytokine responses similar to those seen with human respiratory syncytial virus", journal= "Journal of General Virology", year = "2010", volume = "91", number = "5", pages = "1302-1310", doi = "https://doi.org/10.1099/vir.0.015396-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.015396-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Human metapneumovirus (hMPV) is a major cause of upper and lower respiratory-tract infection in infants, the elderly and immunocompromised individuals. Virus-directed cellular immunity elicited by hMPV infection is poorly understood, in contrast to the phylogenetically and clinically related pathogen human respiratory syncytial virus (hRSV). In a murine model of acute lower respiratory-tract infection with hMPV, we demonstrate the accumulation of gamma interferon (IFN-γ)-producing CD8+ T cells in the airways and lungs at day 7 post-infection (p.i.), associated with cytotoxic T lymphocytes (CTLs) directed to an epitope of the M2-1 protein. This CTL immunity was accompanied by increased pulmonary expression of Th1 cytokines IFN-γ and interleukin (IL)-12 and antiviral cytokines (IFN-β), as well as chemokines Mip-1α, Mip-1β, Mig, IP-10 and CX3CL1. There was also a moderate increase in Th2-type cytokines IL-4 and IL-10 compared with uninfected mice. At 21 days p.i., a strong CTL response could be recalled from the spleen. A similar pattern of CTL induction to the homologous M2-1 CTL epitope of hRSV, and of cytokine/chemokine induction, was observed following infection with hRSV, highlighting similarities in the cellular immune response to the two related pathogens.", }