Novel subtype C human immunodeficiency virus type 1 envelopes cloned directly from plasma: coreceptor usage and neutralization phenotypes
Koh, Willie W. L. and Forsman, Anna and Hué, Stéphane and van der Velden, Gisela J. and Yirrell, David L. and McKnight, Áine and Weiss, Robin A. and Aasa-Chapman, Marlén M. I.,, 91, 2374-2380 (2010),
doi = https://doi.org/10.1099/vir.0.022228-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Human immunodeficiency virus type 1 (HIV-1) is classified into different phylogenetic subtypes, with subtype C representing more than half of the novel infections globally. However, there are relatively few subtype C envelopes available for study. We amplified 18 unique env genes from 13 patients who were infected with subtype C HIV-1 in six African countries and in Scotland to create replication-competent viruses. These envelopes are phylogenetically diverse across the subtype C spectrum, and have on average more N-linked glycosylation sites and slightly longer variable loops than previously described C envelopes. We found that CCR3 coreceptor usage is less prevalent in subtype C than in subtype B viruses, and these envelopes have varied sensitivity to neutralization. The subtype C chimeric viruses generated in this study will be useful for evaluating the breadth of neutralizing antibodies and other entry inhibitors.,
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