1887

Journal of General Virology header logo

Volume 92, Issue 3

Completion of the full-length genome sequence of the infectious salmon anemia virus, an aquatic orthomyxovirus-like, and characterization of mAbs Free

Abstract

We report here the first full-length sequence of the eight ssRNA genome segments of the infectious salmon anemia virus (ISAV, Glesvaer/2/90 isolate), a salmonid orthomyxovirus-like. Comparison of ISAV genome sequence with those of others orthomyxovirus reveals low identity values, and a remarkable feature is the extremely long 5′ end UTR of ISAV segments, which all contain an additional conserved motif of unknown function. In addition to the genome nucleotide sequence determination, specific mAbs have been produced through mice immunization with sucrose-purified ISAV. Four mAbs directed against the haemagglutinin-esterase glycoprotein, the nucleoprotein and free or actin-associated forms of the matrix protein have been characterized by (i) indirect fluorescent antibody test; (ii) virus neutralization; (iii) radioimmunoprecipitation and (iv) Western blot assays. These mAbs will potentially be useful for the development of new diagnostic tests, and the nucleotide sequences will help to establish a reverse genetics system for ISAV.

  • Received:
  • Accepted:
  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.027417-0
2011-03-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/92/3/528.html?itemId=/content/journal/jgv/10.1099/vir.0.027417-0&mimeType=html&fmt=ahah

References

  1. Aspehaug, V., Falk, K., Krossoy, B., Thevarajan, J., Sanders, L., Moore, L., Endresen, C. & Biering, E.(2004). Infectious salmon anemia virus (ISAV) genomic segment 3 encodes the viral nucleoprotein (NP), an RNA-binding protein with two monopartite nuclear localization signals (NLS). Virus Res 106, 51–60.[CrossRef] [Google Scholar]
  2. Aspehaug, V., Mikalsen, A. B., Snow, M., Biering, E. & Villoing, S.(2005). Characterization of the infectious salmon anemia virus fusion protein. J Virol 79, 12544–12553.[CrossRef] [Google Scholar]
  3. Biacchesi, S., Le Berre, M., Le Guillou, S., Benmansour, A., Bremont, M., Quillet, E. & Boudinot, P.(2007). Fish genotype significantly influences susceptibility of juvenile rainbow trout, Oncorhynchus mykiss (Walbaum), to waterborne infection with infectious salmon anaemia virus. J Fish Dis 30, 631–636.[CrossRef] [Google Scholar]
  4. Bierin, E., Falk, K., Hoel, E., Thevarajan, J., Joerink, M., Nylund, A., Endresen, C. & Krossoy, B.(2002). Segment 8 encodes a structural protein of infectious salmon anaemia virus (ISAV); the co-linear transcript from segment 7 probably encodes a non-structural or minor structural protein. Dis Aquat Organ 49, 117–122.[CrossRef] [Google Scholar]
  5. Bouchard, D., Keleher, W., Opitz, H. M., Blake, S., Edwards, K. C. & Nicholson, B. L.(1999). Isolation of infectious salmon anemia virus (ISAV) from Atlantic salmon in New Brunswick, Canada. Dis Aquat Organ 35, 131–137.[CrossRef] [Google Scholar]
  6. Clouthier, S. C., Rector, T., Brown, N. E. & Anderson, E. D.(2002). Genomic organization of infectious salmon anaemia virus. J Gen Virol 83, 421–428. [Google Scholar]
  7. Dannevig, B. H., Falk, K. & Namork, E.(1995). Isolation of the causal virus of infectious salmon anaemia (ISA) in a long-term cell line from Atlantic salmon head kidney. J Gen Virol 76, 1353–1359.[CrossRef] [Google Scholar]
  8. Falk, K., Namork, E., Rimstad, E., Mjaaland, S. & Dannevig, B. H.(1997). Characterization of infectious salmon anemia virus, an orthomyxo-like virus isolated from Atlantic salmon (Salmo salar L.). J Virol 71, 9016–9023. [Google Scholar]
  9. Falk, K., Aspehaug, V., Vlasak, R. & Endresen, C.(2004). Identification and characterization of viral structural proteins of infectious salmon anemia virus. J Virol 78, 3063–3071.[CrossRef] [Google Scholar]
  10. Garcia-Rosado, E., Markussen, T., Kileng, O., Baekkevold, E. S., Robertsen, B., Mjaaland, S. & Rimstad, E.(2008). Molecular and functional characterization of two infectious salmon anaemia virus (ISAV) proteins with type I interferon antagonizing activity. Virus Res 133, 228–238.[CrossRef] [Google Scholar]
  11. Godoy, M. G., Aedo, A., Kibenge, M. J., Groman, D. B., Yason, C. V., Grothusen, H., Lisperguer, A., Calbucura, M., Avendano, F. & other authors(2008). First detection, isolation and molecular characterization of infectious salmon anaemia virus associated with clinical disease in farmed Atlantic salmon (Salmo salar) in Chile. BMC Vet Res 4, 28.[CrossRef] [Google Scholar]
  12. Hellebo, A., Vilas, U., Falk, K. & Vlasak, R.(2004). Infectious salmon anemia virus specifically binds to and hydrolyzes 4-O-acetylated sialic acids. J Virol 78, 3055–3062.[CrossRef] [Google Scholar]
  13. Kawaoka, Y., Cox, N. J., Haller, O., Hongo, S., Kaverin, N., Klenk, H. D., Lamb, R. A., McCauley, J., Palese, P. & other authors(2005).Infectious salmon anaemia virus. New York. : Elsevier Academic Press. [Google Scholar]
  14. Kibenge, F. S., Garate, O. N., Johnson, G., Arriagada, R., Kibenge, M. J. & Wadowska, D.(2001). Isolation and identification of infectious salmon anaemia virus (ISAV) from Coho salmon in Chile. Dis Aquat Organ 45, 9–18.[CrossRef] [Google Scholar]
  15. Kibenge, F. S., Xu, H., Kibenge, M. J., Qian, B. & Joseph, T.(2007). Characterization of gene expression on genomic segment 7 of infectious salmon anaemia virus. Virol J 4, 34.[CrossRef] [Google Scholar]
  16. Kristiansen, M., Froystad, M. K., Rishovd, A. L. & Gjoen, T.(2002). Characterization of the receptor-destroying enzyme activity from infectious salmon anaemia virus. J Gen Virol 83, 2693–2697. [Google Scholar]
  17. Krossoy, B., Hordvik, I., Nilsen, F., Nylund, A. & Endresen, C.(1999). The putative polymerase sequence of infectious salmon anemia virus suggests a new genus within the Orthomyxoviridae. J Virol 73, 2136–2142. [Google Scholar]
  18. Krossoy, B., Devold, M., Sanders, L., Knappskog, P. M., Aspehaug, V., Falk, K., Nylund, A., Koumans, S., Endresen, C. & Biering, E.(2001). Cloning and identification of the infectious salmon anaemia virus haemagglutinin. J Gen Virol 82, 1757–1765. [Google Scholar]
  19. Lovely, J. E., Dannevig, B. H., Falk, K., Hutchin, L., MacKinnon, A. M., Melville, K. J., Rimstad, E. & Griffiths, S. G.(1999). First identification of infectious salmon anaemia virus in North America with haemorrhagic kidney syndrome. Dis Aquat Organ 35, 145–148.[CrossRef] [Google Scholar]
  20. Markussen, T., Jonassen, C. M., Numanovic, S., Braaen, S., Hjortaas, M., Nilsen, H. & Mjaaland, S.(2008). Evolutionary mechanisms involved in the virulence of infectious salmon anaemia virus (ISAV), a piscine orthomyxovirus. Virology 374, 515–527.[CrossRef] [Google Scholar]
  21. McBeath, A. J., Collet, B., Paley, R., Duraffour, S., Aspehaug, V., Biering, E., Secombes, C. J. & Snow, M.(2006). Identification of an interferon antagonist protein encoded by segment 7 of infectious salmon anaemia virus. Virus Res 115, 176–184.[CrossRef] [Google Scholar]
  22. Mjaaland, S., Rimstad, E., Falk, K. & Dannevig, B. H.(1997). Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost. J Virol 71, 7681–7686. [Google Scholar]
  23. Moriette, C., LeBerre, M., Boscher, S. K., Castric, J. & Bremont, M.(2005). Characterization and mapping of monoclonal antibodies against the Sleeping disease virus, an aquatic alphavirus. J Gen Virol 86, 3119–3127.[CrossRef] [Google Scholar]
  24. Raynard, R. S., Murray, A. G. & Gregory, A.(2001). Infectious salmon anaemia virus in wild fish from Scotland. Dis Aquat Organ 46, 93–100.[CrossRef] [Google Scholar]
  25. Rimstad, E., Mjaaland, S., Snow, M., Mikalsen, A. B. & Cunningham, C. O.(2001). Characterization of the infectious salmon anemia virus genomic segment that encodes the putative hemagglutinin. J Virol 75, 5352–5356.[CrossRef] [Google Scholar]
  26. Ritchie, R. J., Heppell, J., Cook, M. B., Jones, S. & Griffiths, S. G.(2001). Identification and characterization of segments 3 and 4 of the ISAV genome. Virus Genes 22, 289–297.[CrossRef] [Google Scholar]
  27. Ritchie, R. J., Bardiot, A., Melville, K., Griffiths, S., Cunningham, C. O. & Snow, M.(2002). Identification and characterisation of the genomic segment 7 of the infectious salmon anaemia virus genome. Virus Res 84, 161–170.[CrossRef] [Google Scholar]
  28. Rowley, H. M., Campbell, S. J., Curran, W. L., Turnbull, T. & Bryson, D. G.(1999). Isolation of infectious salmon anaemia virus (ISAV) from Scottish farmed Atlantic salmon. J Fish Dis 22, 483–487.[CrossRef] [Google Scholar]
  29. Sandvik, T., Rimstad, E. & Mjaaland, S.(2000). The viral RNA 3′- and 5′-end structure and mRNA transcription of infectious salmon anaemia virus resemble those of influenza viruses. Arch Virol 145, 1659–1669.[CrossRef] [Google Scholar]
  30. Snow, M. & Cunningham, C. O.(2001). Characterisation of the putative nucleoprotein gene of infectious salmon anaemia virus (ISAV). Virus Res 74, 111–118.[CrossRef] [Google Scholar]
  31. Snow, M., Ritchie, R., Arnaud, O., Villoing, S., Aspehaug, V. & Cunningham, C. O.(2003). Isolation and characterisation of segment 1 of the infectious salmon anaemia virus genome. Virus Res 92, 99–105.[CrossRef] [Google Scholar]
  32. Thorud, K. & Djupvik, H. O.(1988). Infectious anaemia in Atlantic salmon (Salmo salar L.). Bull Eur Assoc Fish Pathol 8, 109–111. [Google Scholar]
  33. Wergeland, H. & Jakobsen, R.(2001). A salmonid cell line (TO) for production of infectious salmon anaemia virus (ISAV). Dis Aquat Organ 44, 183–190.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.027417-0
Loading
Completion of the full-length genome sequence of the infectious salmon anemia virus, an aquatic orthomyxovirus-like, and characterization of mAbs
J. Gen. Virol. 92, 528 (2011); https://doi.org/10.1099/vir.0.027417-0
/content/journal/jgv/10.1099/vir.0.027417-0
/content/journal/jgv/10.1099/vir.0.027417-0
Loading

Data & Media loading...

Supplements

vol. , part 3, pp. 528 - 533

Genomic segments and encoded proteins of ISAV versus IAV

Primers used for molecular cloning [Single PDF file](44 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error