1887

Abstract

Baculoviruses infect their lepidopteran hosts via the midgut epithelium through binding of occlusion-derived virus (ODV) and fusion between the virus envelope and microvillar membranes. To identify genes and sequences that are involved in this process, a random phage display library was screened for peptides that bound to brush border membrane vesicles (BBMV) derived from the midgut epithelium of . Seventeen peptides that bound to BBMV were recovered. Two of these, HV1 and HV2, had sequence similarity to the ODV envelope protein ODV-E66 that is found in five species of alphabaculoviruses. Chemically synthesized versions of HV1 and HV2, and two peptides (E66A and E66B) derived from similar sequences of multiple nucleopolyhedrovirus (MNPV) ODV-E66, bound to unfixed cryosections of whole midgut tissues. E66A, but not HV1, bound to gut BBMV proteins on a far-Western blot. Competition assays with HV1 and purified MNPV ODV resulted in decreased mortality of larvae at a dose of 1 LD, and a significant increase in survival time at higher virus concentrations. These results suggest a role for ODV-E66 in baculovirus infection of lepidopteran larval midgut epithelium.

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2011-05-01
2024-03-28
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