RT Journal Article SR Electronic(1) A1 Bouvaine, Sophie A1 Boonham, Neil A1 Douglas, Angela E.YR 2011 T1 Interactions between a luteovirus and the GroEL chaperonin protein of the symbiotic bacterium Buchnera aphidicola of aphids JF Journal of General Virology, VO 92 IS 6 SP 1467 OP 1474 DO https://doi.org/10.1099/vir.0.029355-0 PB Microbiology Society, SN 1465-2099, AB Luteoviruses and poleroviruses are important plant viruses transmitted exclusively by aphids in a circulative manner via the aphid haemolymph. A chaperonin protein, GroEL, synthesized in aphids by a symbiotic bacterium, Buchnera aphidicola, is hypothesized to bind to virus particles in the haemolymph, thereby promoting transmission. To investigate this hypothesis, the GroEL-binding site for barley yellow dwarf virus (BYDV) was determined in vitro, and the abundance of GroEL protein in different aphid tissues was investigated. Virus binding to a peptide library representing the full GroEL molecule revealed a single binding site that coincides with the site that anchors two GroEL rings to form the native GroEL tetradecamer. In the functional form of the GroEL protein, virus binding would compete with the formation of the two GroEL rings. Using a mAb raised against a Buchnera-specific GroEL epitope, GroEL was detected in Buchnera cells by immunoblotting and immunocytochemistry, but not in the aphid haemolymph, fat body or gut. From the prediction here that GroEL–virus interactions are probably severely limited by competition with other GroEL molecules, and the evidence that GroEL is not available to interact with virus particles in vivo, it is concluded that GroEL–virus interactions are unlikely to contribute to virus transmission by aphids., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.029355-0