%0 Journal Article %A Köhler, Anja %A Gottschling, Marc %A Manning, Kizzie %A Lehmann, Mandy D. %A Schulz, Eric %A Krüger-Corcoran, Daniela %A Stockfleth, Eggert %A Nindl, Ingo %T Genomic characterization of ten novel cutaneous human papillomaviruses from keratotic lesions of immunosuppressed patients %D 2011 %J Journal of General Virology, %V 92 %N 7 %P 1585-1594 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.030593-0 %I Microbiology Society, %X Viral warts from immunosuppressed organ transplant recipients (OTR) persist over years and may progress into non-melanoma skin cancer. The types of human papillomaviruses (HPV) in such lesions are different from that seen in the general population. A subset of these lesions is not infected with the classical wart-associated HPV types. In order to gain a better understanding of the HPV types in those lesions, we isolated ten novel HPVs from persisting keratotic lesions of immunosuppressed OTRs by rolling circle amplification and subsequent long-template PCR. Additionally, we sequenced and characterized the whole genome of the ten novel HPV types. Phylogenetic analyses revealed that nine HPV types belonged to the genus Gammapapillomavirus (γ-PV) and one to the genus Betapapillomavirus. In a phylogenetic analysis using L1 fragments of human and non-human PV types, primate papillomaviruses and our novel HPV types nested within the genus γ-PV in a highly polyphyletic pattern. This study significantly broadens the knowledge concerning the diversity and evolution of the poorly known γ-PV types. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.030593-0