Three-dimensional reconstruction of Heterocapsa circularisquama RNA virus by electron cryo-microscopy
Miller, Jennifer L. and Woodward, Jeremy and Chen, Shaoxia and Jaffer, Mohammed and Weber, Brandon and Nagasaki, Keizo and Tomaru, Yuji and Wepf, Roger and Roseman, Alan and Varsani, Arvind and Sewell, Trevor,, 92, 1960-1970 (2011),
doi = https://doi.org/10.1099/vir.0.031211-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Heterocapsa circularisquama RNA virus is a non-enveloped icosahedral ssRNA virus infectious to the harmful bloom-forming dinoflagellate, H. circularisquama, and which is assumed to be the major natural agent controlling the host population. The viral capsid is constructed from a single gene product. Electron cryo-microscopy revealed that the virus has a diameter of 34 nm and T = 3 symmetry. The 180 quasi-equivalent monomers have an unusual arrangement in that each monomer contributes to a ‘bump’ on the surface of the protein. Though the capsid protein probably has the classic ‘jelly roll’ β-sandwich fold, this is a new packing arrangement and is distantly related to the other positive-sense ssRNA virus capsid proteins. The handedness of the structure has been determined by a novel method involving high resolution scanning electron microscopy of the negatively stained viruses and secondary electron detection.,
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