1887

Abstract

Human hepatitis E virus (HEV) is considered an emerging pathogen in industrialized countries. The aim of the present study was to contribute to the body of knowledge available on the molecular epidemiology of acute hepatitis E in Italy. Three sets of HEV-specific primers targeting the ORF1 and ORF2 were used to examine serum samples collected from acute hepatitis patients positive for anti-HEV IgG and/or IgM, between 2007 and 2010. Seventeen patients (39.5 %) tested HEV RNA-positive: 12 infections, due to genotype 1, were associated with travel to endemic areas (Bangladesh, India and Pakistan), while five infections, due to genotype 3, were presumably autochthonous. Risk factors identified in this group included exposure to raw seafood, pork liver sausages and wild boar. Results from the present study confirm that human HEV infection in Italy is caused by different genotypes, depending on whether the infection is travel-related or autochthonous.

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2011-07-01
2024-03-29
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References

  1. Aggarwal R. 2011; Hepatitis E: historical, contemporary and future perspectives. J Gastroenterol Hepatol 26:Suppl. 172–82 [View Article][PubMed]
    [Google Scholar]
  2. Aggarwal R., Kini D., Sofat S., Naik S. R., Krawczynski K. 2000; Duration of viraemia and faecal viral excretion in acute hepatitis E. Lancet 356:1081–1082 [View Article][PubMed]
    [Google Scholar]
  3. Alavian S., Fallahian F., Lankarani K. 2009; Epidemiology of hepatitis E in Iran and Pakistan. Hepat Mon 9:60–65
    [Google Scholar]
  4. Bartolini B., Garbuglia A. R., Horejsh D., Martini F., Carletti F., Capobianchi M. R. 2007; Simultaneous control of DNA and RNA processing efficiency using a nucleic acid calibration set. Biotechniques 42:452–456 [View Article][PubMed]
    [Google Scholar]
  5. Cacopardo B., Russo R., Preiser W., Benanti F., Brancati G., Nunnari A. 1997; Acute hepatitis E in Catania (eastern Sicily) 1980–1994. The role of hepatitis E virus. Infection 25:313–316 [View Article][PubMed]
    [Google Scholar]
  6. Caprioli A., Martelli F., Ostanello F., Di Bartolo I., Ruggeri F. M., Del Chiaro L., Tolari F. 2007; Detection of hepatitis E virus in Italian pig herds. Vet Rec 161:422–423 [View Article][PubMed]
    [Google Scholar]
  7. Clemente-Casares P., Rodriguez-Manzano J., Girones R. 2009; Hepatitis E virus genotype 3 and sporadically also genotype 1 circulate in the population of Catalonia, Spain. J Water Health 7:664–673 [View Article][PubMed]
    [Google Scholar]
  8. Dalton H. R., Thurairajah P. H., Fellows H. J., Hussaini H. S., Mitchell J., Bendall R., Banks M., Ijaz S., Teo C. G., Levine D. F. 2007; Autochthonous hepatitis E in southwest England. J Viral Hepat 14:304–309 [View Article][PubMed]
    [Google Scholar]
  9. Di Bartolo I., Martelli F., Inglese N., Pourshaban M., Caprioli A., Ostanello F., Ruggeri F. M. 2008; Widespread diffusion of genotype 3 hepatitis E virus among farming swine in Northern Italy. Vet Microbiol 132:47–55 [View Article][PubMed]
    [Google Scholar]
  10. Di Bartolo I., Ponterio E., Castellini L., Ostanello F., Ruggeri F. M. 2011; Viral and antibody HEV prevalence in swine at slaughterhouse in Italy. Vet Microbiol 149:330–338[PubMed] [CrossRef]
    [Google Scholar]
  11. Fogeda M., Avellón A., Cilla C. G., Echevarría J. M. 2009; Imported and autochthonous hepatitis E virus strains in Spain. J Med Virol 81:1743–1749 [View Article][PubMed]
    [Google Scholar]
  12. Grieco A., Miele L., Gasbarrini G., Grillo R. 2001; Sporadic HEV hepatitis in Italy. Gut 48:580 [View Article][PubMed]
    [Google Scholar]
  13. Herremans M., Bakker J., Duizer E., Vennema H., Koopmans M. P. G. 2007; Use of serological assays for diagnosis of hepatitis E virus genotype 1 and 3 infections in a setting of low endemicity. Clin Vaccine Immunol 14:562–568 [View Article][PubMed]
    [Google Scholar]
  14. Huang S., Zhang X., Jiang H., Yan Q., Ai X., Wang Y., Cai J., Jiang L., Wu T. et al. 2010; Profile of acute infectious markers in sporadic hepatitis E. PLoS ONE 5:e13560 [View Article][PubMed]
    [Google Scholar]
  15. Kaba M., Davoust B., Marié J. L., Colson P. 2010; Detection of hepatitis E virus in wild boar (Sus scrofa) livers. Vet J 186:259–261 [View Article][PubMed]
    [Google Scholar]
  16. Labrique A. B., Zaman K., Hossain Z., Saha P., Yunus M., Hossain A., Ticehurst J., Nelson K. E. 2009; Population seroprevalence of hepatitis E virus antibodies in rural Bangladesh. Am J Trop Med Hyg 81:875–881 [View Article][PubMed]
    [Google Scholar]
  17. La Rosa G., Pourshaban M., Iaconelli M., Spuri-Vennarucci V., Muscillo M. 2010; Molecular detection of hepatitis E virus from sewage. Arch Virol 155:589–593[PubMed] [CrossRef]
    [Google Scholar]
  18. Legrand-Abravanel F., Thevenet I., Mansuy J. M., Saune K., Vischi F., Peron J. M., Kamar N., Rostaing L., Izopet J. 2009; Good performance of immunoglobulin M assays in diagnosing genotype 3 hepatitis E virus infections. Clin Vaccine Immunol 16:772–774 [View Article][PubMed]
    [Google Scholar]
  19. Lewis H. C., Wichmann O., Duizer E. 2010a; Transmission routes and risk factors for autochthonous hepatitis E virus infection in Europe: a systematic review. Epidemiol Infect 138:145–166 [View Article][PubMed]
    [Google Scholar]
  20. Lewis H. C., Wichmann O., Duizer E. 2010b; Transmission routes and risk factors for autochthonous hepatitis E virus infection in Europe: a systematic review. Epidemiol Infect 138:145–166 [View Article][PubMed]
    [Google Scholar]
  21. Martelli F., Toma S., Di Bartolo I., Caprioli A., Ruggeri F. M., Lelli D., Bonci M., Ostanello F. 2010; Detection of Hepatitis E Virus (HEV) in Italian pigs displaying different pathological lesions. Res Vet Sci 88:492–496 [View Article][PubMed]
    [Google Scholar]
  22. Masia G., Orrù G., Liciardi M., Desogus G., Coppola R. C., Murru V., Argiolas M., Orrù G. 2009; Evidence of hepatitis E virus (HEV) infection in human and pigs in Sardinia, Italy. J Prev Med Hyg 50:227–231[PubMed]
    [Google Scholar]
  23. Mathur P., Arora N. K., Panda S. K., Kapoor S. K., Jailkhani B. L., Irshad M. 2001; Sero-epidemiology of hepatitis E virus (HEV) in urban and rural children of North India. Indian Pediatr 38:461–475[PubMed]
    [Google Scholar]
  24. McCaustland K. A., Bi S., Purdy M. A., Bradley D. W. 1991; Application of two RNA extraction methods prior to amplification of hepatitis E virus nucleic acid by the polymerase chain reaction. J Virol Methods 35:331–342 [View Article][PubMed]
    [Google Scholar]
  25. Meng X. J. 2010; Recent advances in hepatitis E virus. J Viral Hepat 17:153–161[PubMed] [CrossRef]
    [Google Scholar]
  26. Mizuo H., Yazaki Y., Sugawara K., Tsuda F., Takahashi M., Nishizawa T., Okamoto H. 2005; Possible risk factors for the transmission of hepatitis E virus and for the severe form of hepatitis E acquired locally in Hokkaido, Japan. J Med Virol 76:341–349 [View Article][PubMed]
    [Google Scholar]
  27. Mushahwar I. K. 2008; Hepatitis E virus: molecular virology, clinical features, diagnosis, transmission, epidemiology, and prevention. J Med Virol 80:646–658 [View Article][PubMed]
    [Google Scholar]
  28. Panda S. K., Thakral D., Rehman S. 2007; Hepatitis E virus. Rev Med Virol 17:151–180 [View Article][PubMed]
    [Google Scholar]
  29. Pavio N., Meng X. J., Renou C. 2010; Zoonotic hepatitis E: animal reservoirs and emerging risks. Vet Res 41:46 [View Article][PubMed]
    [Google Scholar]
  30. Pelosi E., Clarke I. 2008; Hepatitis E: a complex and global disease. Emerg Health Threats J 1:
    [Google Scholar]
  31. Pina S., Buti M., Cotrina M., Piella J., Girones R. 2000; HEV identified in serum from humans with acute hepatitis and in sewage of animal origin in Spain. J Hepatol 33:826–833 [View Article][PubMed]
    [Google Scholar]
  32. Renou C., Moreau X., Pariente A., Cadranel J. F., Maringe E., Morin T., Causse X., Payen J. L., Izopet J. et al. 2008; A national survey of acute hepatitis E in France. Aliment Pharmacol Ther 27:1086–1093 [View Article][PubMed]
    [Google Scholar]
  33. Renou C., Nicand E., Pariente A., Cadranel J. F., Pavio N. 2009; Quand rechercher et comment diagnostiquer une hépatite E autochtone?. Gastroenterol Clin Biol 33:Suppl.F27–F35 (in French) [View Article][PubMed]
    [Google Scholar]
  34. Rodriguez-Manzano J., Miagostovich M., Hundesa A., Clemente-Casares P., Carratala A., Buti M., Jardi R., Girones R., Hundesa A. et al. 2010; Analysis of the evolution in the circulation of HAV and HEV in eastern Spain by testing urban sewage samples. J Water Health 8:346–354 [View Article][PubMed]
    [Google Scholar]
  35. Romanò L., Paladini S., Tagliacarne C., Canuti M., Bianchi S., Zanetti A. R. 2011; Hepatitis E in Italy: a long-term prospective study. J Hepatol 54:34–40 [View Article][PubMed]
    [Google Scholar]
  36. Sadler G. J., Mells G. F., Shah N. H., Chesner I. M., Walt R. P. 2006; UK acquired hepatitis E–an emerging problem?. J Med Virol 78:473–475 [View Article][PubMed]
    [Google Scholar]
  37. Schielke A., Sachs K., Lierz M., Appel B., Jansen A., Johne R. 2009; Detection of hepatitis E virus in wild boars of rural and urban regions in Germany and whole genome characterization of an endemic strain. Virol J 6:58 [View Article][PubMed]
    [Google Scholar]
  38. Schlauder G. G., Desai S. M., Zanetti A. R., Tassopoulos N. C., Mushahwar I. K. 1999; Novel hepatitis E virus (HEV) isolates from Europe: evidence for additional genotypes of HEV. J Med Virol 57:243–251 [View Article][PubMed]
    [Google Scholar]
  39. Shrestha S. M., Shrestha S., Tsuda F., Nishizawa T., Gotanda Y., Takeda N., Okamoto H. 2003; Molecular investigation of hepatitis E virus infection in patients with acute hepatitis in Kathmandu, Nepal. J Med Virol 69:207–214 [View Article][PubMed]
    [Google Scholar]
  40. Teo C. G. 2007; The two clinico-epidemiological forms of hepatitis E. J Viral Hepat 14:295–297 [View Article][PubMed]
    [Google Scholar]
  41. Zanetti A. R., Schlauder G. G., Romanò L., Tanzi E., Fabris P., Dawson G. J., Mushahwar I. K. 1999; Identification of a novel variant of hepatitis E virus in Italy. J Med Virol 57:356–360 [View Article][PubMed]
    [Google Scholar]
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