Construction of gene transfer vectors based on simian adenovirus 7 Roy, Soumitra and Clawson, David S. and Adam, Virginie S. and Medina, Angelica and Wilson, James M.,, 92, 1749-1753 (2011), doi = https://doi.org/10.1099/vir.0.032300-0, publicationName = Microbiology Society, issn = 0022-1317, abstract= The complete nucleotide sequence of an isolate of simian adenovirus 7 (SAdV-7) was determined. The genome organization of this isolate was found to be similar to that of other primate adenoviruses with two principal notable points: severe truncation of the E1A and E1B 19K proteins and an E3 region encoding only the 12.5K homologue. The viral gene products of SAdV-7 are most closely related to simian adenovirus 1 (SAdV-1), and like SAdV-1, are related to the human adenovirus species HAdV-F, such as the enteric adenoviruses HAdV-40 and HAdV-41 and the recently defined HAdV-G (HAdV-52). Two kinds of gene transfer vectors were made: a replication-competent SAdV-7-based vector with no genomic deletion, and a standard replication-incompetent vector deleted for E1. Importantly, the E1-deleted vector could be propagated to high titre by trans-complementation in human HEK 293 cells., language=, type=