1887

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Volume 93, Issue 1

A cross-protective mAb recognizes a novel epitope within the flavivirus NS1 protein Free

Abstract

Despite a resurgence of flavivirus infections worldwide, no approved therapeutic agent exists for any member of the genus. While cross-reactive antibodies with therapeutic potential against flaviviruses have been generated, the majority of them are anti-E antibodies with the potential to cause antibody-dependent enhancement of flavivirus infection and disease. We described previously mAbs against the non-structural NS1 protein of the West Nile virus (WNV) that were protective in mice when administered pre- or post-infection of WNV. Here, we demonstrate that one of these mAbs (16NS1) cross-reacted with Japanese encephalitis virus (JEV) and exhibited protective activity against a lethal JEV infection. Overlapping peptide mapping analysis combined with site-specific mutations identified a novel epitope KAWGKSILFA and critical amino acid residues (W and I) for 16NS1 mAb binding. These results may facilitate the development of a broadly therapeutic mAb that lacks enhancing potential and/or subunit-based vaccine against flaviviruses that target the NS1 protein.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
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2012-01-01
2024-04-20
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A cross-protective mAb recognizes a novel epitope within the flavivirus NS1 protein
J. Gen. Virol. 93, 20 (2012); https://doi.org/10.1099/vir.0.036640-0
/content/journal/jgv/10.1099/vir.0.036640-0
/content/journal/jgv/10.1099/vir.0.036640-0
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