@article{mbs:/content/journal/jgv/10.1099/vir.0.037838-0, author = "Kim, Hye Kwon and Park, Seong Jun and Nguyen, Van Giap and Song, Dae Sub and Moon, Hyoung Joon and Kang, Bo Kyu and Park, Bong Kyun", title = "Identification of a novel single-stranded, circular DNA virus from bovine stool", journal= "Journal of General Virology", year = "2012", volume = "93", number = "3", pages = "635-639", doi = "https://doi.org/10.1099/vir.0.037838-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.037838-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "We report the identification of a novel single-stranded, circular DNA virus isolated from bovine stool. The virus, named bovine stool-associated circular DNA virus (BoSCV), has a genome comprising 2600 bases of circular ssDNA, with two putative ORFs encoding replicase and capsid proteins, arranged inversely. The stem–loop structure was located between the 3′ ends of the two putative ORFs, as in chimpanzee stool-associated circular virus (ChimpSCV) and unlike other circular DNA viruses, including members of the families Circoviridae, Nanoviridae and Geminiviridae. BoSCV was also genetically similar to ChimpSCV, with approximately 30 % identity in the replicase and capsid proteins. A phylogenetic analysis based on the replicase protein showed that BoSCV and ChimpSCV are in the same clade. A field survey using BoSCV-specific PCRs targeting ORF1 detected BoSCV and BoSCV-like sequences in bovine and porcine stool samples. BoSCV appears to belong to a new genus of circular DNA viruses.", }