RT Journal Article SR Electronic(1) A1 Kim, Hye Kwon A1 Park, Seong Jun A1 Nguyen, Van Giap A1 Song, Dae Sub A1 Moon, Hyoung Joon A1 Kang, Bo Kyu A1 Park, Bong KyunYR 2012 T1 Identification of a novel single-stranded, circular DNA virus from bovine stool JF Journal of General Virology, VO 93 IS 3 SP 635 OP 639 DO https://doi.org/10.1099/vir.0.037838-0 PB Microbiology Society, SN 1465-2099, AB We report the identification of a novel single-stranded, circular DNA virus isolated from bovine stool. The virus, named bovine stool-associated circular DNA virus (BoSCV), has a genome comprising 2600 bases of circular ssDNA, with two putative ORFs encoding replicase and capsid proteins, arranged inversely. The stem–loop structure was located between the 3′ ends of the two putative ORFs, as in chimpanzee stool-associated circular virus (ChimpSCV) and unlike other circular DNA viruses, including members of the families Circoviridae, Nanoviridae and Geminiviridae. BoSCV was also genetically similar to ChimpSCV, with approximately 30 % identity in the replicase and capsid proteins. A phylogenetic analysis based on the replicase protein showed that BoSCV and ChimpSCV are in the same clade. A field survey using BoSCV-specific PCRs targeting ORF1 detected BoSCV and BoSCV-like sequences in bovine and porcine stool samples. BoSCV appears to belong to a new genus of circular DNA viruses., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.037838-0