RT Journal Article SR Electronic(1) A1 Xu, Yi-Peng A1 Gu, Lin-Zhu A1 Lou, Yi-Han A1 Cheng, Ruo-Lin A1 Xu, Hai-Jun A1 Wang, Wen-Bing A1 Zhang, Chuan-XiYR 2012 T1 A baculovirus isolated from wild silkworm encompasses the host ranges of Bombyx mori nucleopolyhedrosis virus and Autographa californica multiple nucleopolyhedrovirus in cultured cells JF Journal of General Virology, VO 93 IS 11 SP 2480 OP 2489 DO https://doi.org/10.1099/vir.0.043836-0 PB Microbiology Society, SN 1465-2099, AB A baculovirus, named BomaNPV S2, was isolated from a diseased larva of the wild silkworm, Bombyx mandarina. Notably, BomaNPV S2 exhibited a distinguishing feature in that its host range covered that of both Bombyx mori nucleopolyhedrosis virus (BmNPV) and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) in cultured cells. It could replicate in cells of B. mori (Bm5 and BmN), Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn-5B1-4). However, occlusion-derived virions of BomaNPV S2 in B. mori cells contained only a single nucleocapsid, whereas they contained multiple nucleocapsids in Tn-5B1-4 cells. The complete genome sequence of BomaNPV S2, including predicted ORFs, was determined and compared with the genome sequence of its close relatives. The comparison results showed that most of the BomaNPV S2 genome sequence was shared with BmNPV (BmNPV T3) or BomaNPV S1, but several regions seemed more similar to regions of AcMNPV. This observation might explain why BomaNPV S2 covers the host ranges of BmNPV and AcMNPV. Further recombinant virus infection experiments demonstrated that GP64 plays an important role in BomaNPV S2 host-range determination., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.043836-0