Creation of a completely helper cell-dependent recombinant morbillivirus
Baron, Jana and Baron, Michael,, 94, 1195-1199 (2013),
doi = https://doi.org/10.1099/vir.0.050872-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= We have created a completely helper cell-dependent morbillivirus by modifying the genome to remove the coding sequence of the phosphoprotein (P) and recovering the recombinant virus in a cell line constitutively expressing the P protein. The P protein-deleted virus (P−) grew very inefficiently unless both of the viral accessory proteins (V and C) were also expressed. Growth of the virus was restricted to the P-expressing cell line. The P− virus grew more slowly than the parental virus and expressed much less viral protein in infected cells. The technique could be used to create virus-like particles for use as a vaccine or as antigen in immunological or serological assays.,
language=,
type=