RNA-binding region of Macrobrachium rosenbergii nodavirus capsid protein
Goh, Zee Hong and Mohd, Nur Azmina Syakirin and Tan, Soon Guan and Bhassu, Subha and Tan, Wen Siang,, 95, 1919-1928 (2014),
doi = https://doi.org/10.1099/vir.0.064014-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= White tail disease (WTD) kills prawn larvae and causes drastic losses to the freshwater prawn (Macrobrachium rosenbergii) industry. The main causative agent of WTD is Macrobrachium rosenbergii nodavirus (MrNV). The N-terminal end of the MrNV capsid protein is very rich in positively charged amino acids and is postulated to interact with RNA molecules. N-terminal and internal deletion mutagenesis revealed that the RNA-binding region is located at positions 20–29, where 80 % of amino acids are positively charged. Substitution of all these positively charged residues with alanine abolished the RNA binding. Mutants without the RNA-binding region still assembled into virus-like particles, suggesting that this region is not a part of the capsid assembly domain. This paper is, to the best of our knowledge, the first to report the specific RNA-binding region of MrNV capsid protein.,
language=,
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