Single-stranded positive-sense RNA viruses generated in days using infectious subgenomic amplicons
Aubry, Fabien and Nougairède, Antoine and de Fabritus, Lauriane and Querat, Gilles and Gould, Ernest A. and de Lamballerie, Xavier,, 95, 2462-2467 (2014),
doi = https://doi.org/10.1099/vir.0.068023-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Reverse genetics is a key methodology for producing genetically modified RNA viruses and deciphering cellular and viral biological properties, but methods based on the preparation of plasmid-based complete viral genomes are laborious and unpredictable. Here, both wild-type and genetically modified infectious RNA viruses were generated in days using the newly described ISA (infectious-subgenomic-amplicons) method. This new versatile and simple procedure may enhance our capacity to obtain infectious RNA viruses from PCR-amplified genetic material.,
language=,
type=