1887

Abstract

An accidental intra- and interspecies transmission of scrapie occurred in Italy in 1997 and 1998 following exposure to a vaccine against . PrP in affected sheep and goats, collected from a single flock exposed to vaccination 2 years earlier, was molecularly typed. In five animals with iatrogenic scrapie, a PrP type with a 20 kDa core fragment was found in all areas of the brain investigated. In three sheep and one goat, this isoform co-occurred with a fully glycosylated isoform that had a protease-resistant backbone of 17 kDa, whereas in two sheep and four goats, the two PrP types were detected in different regions of the brain. In sheep with natural field scrapie, a PrP type with physico-chemical properties indistinguishable from the 20 kDa isoform was found. The present results suggest the co-presence of two prion strains in mammary gland and brain homogenates used for vaccination.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.18774-0
2003-04-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/4/vir841047.html?itemId=/content/journal/jgv/10.1099/vir.0.18774-0&mimeType=html&fmt=ahah

References

  1. Agrimi U., Ru G., Cardone F., Pocchiari M., Caramelli M. 1999; Epidemic of transmissible spongiform encephalopathy in sheep and goats in Italy. Lancet 353:560–561
    [Google Scholar]
  2. Baron T. G. M., Madec J.-Y., Calavas D., Richard Y., Barillet F. 2000; Comparison of French natural scrapie isolates with bovine spongiform encephalopathy and experimental scrapie infected sheep. Neurosci Lett 284:175–178
    [Google Scholar]
  3. Bartz J. C., Bessen R. A., McKenzie D., Marsh R. F., Aiken J. M. 2000; Adaptation and selection of prion protein strain conformations following interspecies transmission of transmissible mink encephalopathy. J Virol 74:5542–5547
    [Google Scholar]
  4. Bessen R. A., Marsh R. F. 1994; Distinct PrP properties suggest the molecular basis of strain variation in transmissible mink encephalopathy. J Virol 68:7859–7868
    [Google Scholar]
  5. Bruce M. E. 1993; Scrapie strain variation and mutation. Br Med Bull 48:822–838
    [Google Scholar]
  6. Bruce M. E., McConnell I., Fraser H., Dickinson A. G. 1991; The disease characteristics of different strains of scrapie in Sinc congenic mouse lines: implications for the nature of the agent and host control of pathogenesis. J Gen Virol 72:595–603
    [Google Scholar]
  7. Bruce M. E., Will R. G., Ironside J. W. 10 other authors 1997; Transmissions to mice indicate that ‘new variant’ CJD is caused by the BSE agent. Nature 389:498–501
    [Google Scholar]
  8. Bruce M. E., Boyle A., Cousens S., McConnell I., Foster J., Goldmann W., Fraser H. 2002; Strain characterization of natural sheep scrapie and comparison with BSE. J Gen Virol 83:695–704
    [Google Scholar]
  9. Capucchio M. T., Guarda F., Isaia M. C., Caracappa S., Di Marco V. 1998; Natural occurrence of scrapie in goats in Italy. Vet Rec 143:452–453
    [Google Scholar]
  10. Capucchio M. T., Guarda F., Pozzato N., Coppolino S., Caracappa S., Di Marco V. 2001; Clinical signs and diagnosis of scrapie in Italy: a comparative study in sheep and goats. J Vet Med A Physiol Pathol Clin Med 48:23–31
    [Google Scholar]
  11. Caramelli M., Ru G., Casalone C., Bozzetta E., Acutis P. L., Calella A., Forloni G. 2001; Evidence for the transmission of scrapie to sheep and goats from a vaccine against Mycoplasma agalactiae . Vet Rec 148:531–536
    [Google Scholar]
  12. Foster J. D., Hope J., Fraser H. 1993; Transmission of bovine spongiform encephalopathy to sheep and goats. Vet Rec 133:339–341
    [Google Scholar]
  13. Goldmann W., Martin T., Foster J., Hughes S., Smith G., Hughes K., Dawson M., Hunter N. 1996; Novel polymorphisms in the caprine PrP gene: a codon 142 mutation associated with scrapie incubation period. J Gen Virol 77:2885–2891
    [Google Scholar]
  14. Hill A. F., Desbruslais M., Joiner S., Sidle K. C. L., Gowland I., Collinge J., Doey L. J., Lantos P. 1997; The same prion strain causes vCJD and BSE. Nature 389:448–450
    [Google Scholar]
  15. Hill A. F., Sidle K. C. L., Joiner S., Keyes P., Martin T. C., Dawson M., Collinge J. 1998; Molecular screening of sheep for bovine spongiform encephalopathy. Neurosci Lett 255:159–162
    [Google Scholar]
  16. Hope J., Wood S. C., Birkett C. R., Chong A., Bruce M. E., Cairns D., Goldmann W., Hunter N., Bostock C. J. 1999; Molecular analysis of ovine prion protein identifies similarities between BSE and an experimental isolate of natural scrapie, CH1641. J Gen Virol 80:1–4 corrigendum 81, 851
    [Google Scholar]
  17. Madec J. Y., Groschup M. H., Calavas D., Junghans F., Baron T. 2000; Protease-resistant prion protein in brain and lymphoid organs of sheep within a naturally scrapie-infected flock. Microb Pathog 28:353–362
    [Google Scholar]
  18. Mechelli L., Mantovani A. 1988; Scrapie in sheep in central and southern Italy. Vet Res Commun 12:165–167
    [Google Scholar]
  19. Parchi P., Giese A., Capellari S. 15 other authors 1999; Classification of sporadic Creutzfeldt–Jakob disease based on molecular and phenotypic analysis of 300 subjects. Ann Neurol 46:224–233
    [Google Scholar]
  20. Prusiner S. B. 1997; Prion diseases and the BSE crisis. Science 278:245–251
    [Google Scholar]
  21. Puoti G., Giaccone G., Rossi G., Canciani B., Bugiani O., Tagliavini F. 1999; Sporadic Creutzfeldt–Jakob disease: co-occurrence of different types of PrPSc in the same brain. Neurology 53:2173–2176
    [Google Scholar]
  22. Vaccari G., Petraroli R., Agrimi U. 8 other authors 2001; PrP genotype in Sarda breed sheep and its relevance to scrapie. Arch Virol 146:2029–2037
    [Google Scholar]
  23. Zanusso G., Liu D., Ferrari S. 11 other authors 1998; Prion protein expression in different species: analysis with a panel of new mAbs. Proc Natl Acad Sci U S A 95:8812–8816
    [Google Scholar]
  24. Zanusso G., Farinazzo A., Fiorini M., Gelati M., Castagna A., Righetti P. G., Rizzuto N., Monaco S. 2001; pH-dependent prion protein conformation in classical Creutzfeldt–Jakob disease. J Biol Chem 276:40377–40380
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.18774-0
Loading
/content/journal/jgv/10.1099/vir.0.18774-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error