1887

Abstract

Using synthetic peptides we have shown that positively charged sequences present at the C terminus of the L1 protein and the N and C termini of the L2 protein of human papillomavirus type 16 (HPV-16) bind to both DNA and heparan sulfate receptors. Moreover, these short amino acid sequences are sufficient to mediate gene transfer in COS-7 cells. The L1 proteins of other HPVs were shown to contain one or two DNA- and heparin-binding sequences that have the capacity to transfer genes. These DNA-binding sequences also recognized the enhancing packaging sequence of bovine papillomavirus type 1. The results suggest that the L2 protein could participate in DNA packaging during maturation of virions.

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2003-01-01
2024-03-28
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References

  1. Baker T. S., Newcomb W. W., Olson N. H., Cowsert L. M., Olson C., Brown J. C. 1991; Structures of bovine and human papillomaviruses. Analysis by cryoelectron microscopy and three-dimensional image reconstruction. Biophys J 60:1445–1456
    [Google Scholar]
  2. Bousarghin L., Touzé A., Combita A.-l., El Mehdaoui S., Sizaret P.-Y., Bravo M. M., Coursaget P. 2002; Detection of neutralizing antibodies against human papillomaviruses (HPV) by inhibition of gene transfer mediated by HPV pseudovirions. J Clin Microbiol 40:926–932
    [Google Scholar]
  3. Chen X. S., Garcea R. L., Goldberg I., Casini G., Harrison S. C. 2000; Structure of the small virus-like particles assembled from the L1 protein of human papillomavirus 16. Mol Cell 5:557–567
    [Google Scholar]
  4. Combita A. L., Touzé A., Bousarghin L., Sizaret P.-Y., Munoz N., Coursaget P. 2001; Gene transfer using human papillomavirus pseudovirions varies according to virus genotype and requires cell surface heparan sulfate. FEMS Microbiol Lett 204:183–188
    [Google Scholar]
  5. El Mehdaoui S., Touzé A., Laurent S., Sizaret P. Y., Rasschaert D., Coursaget P. 2000; Gene transfer using recombinant rabbit hemorrhagic disease virus capsids with genetically modified DNA encapsidation capacity by addition of packaging sequences from L1 or L2 protein of human papillomavirus type 16. J Virol 74:10332–10340
    [Google Scholar]
  6. Evander M., Frazer I. H., Payne E., Qi Y. M., Hengst K., McMillan N. A. 1997; Identification of the α6 integrin as a candidate receptor for papillomaviruses. J Virol 71:2449–2456
    [Google Scholar]
  7. Giroglou T., Florin L., Schafer F., Streeck R. E., Sapp M. 2001; Human papillomavirus infection requires cell surface heparan sulfate. J Virol 75:1565–1570
    [Google Scholar]
  8. Joyce J. G., Tung J. S., Przysieck C. T., Cook J. C., Lehman E. D., Sands J. A., Jansen K. U., Keller P. M. 1999; The L1 major capsid protein of human papillomavirus type 11 recombinant virus-like particles interacts with heparin and cell-surface glycosaminoglycans on human keratinocytes. J Biol Chem 274:5810–5822
    [Google Scholar]
  9. Kawana K., Yoshikawa H., Taketani Y., Yoshiike K., Kanda T. 1998; In vitro construction of pseudovirions of human papillomavirus type 16: incorporation of plasmid DNA into reassembled L1/L2 capsids. J Virol 72:10298–10300
    [Google Scholar]
  10. Kichler A., Pages J. C., Leborgne C. 7 other authors 2000; Efficient DNA transfection mediated by the C-terminal domain of human immunodeficiency virus type 1 viral protein R. J Virol 74:5424–5431
    [Google Scholar]
  11. Kirnbauer R., Taub J., Greenstone H., Roden R., Durst M., Gissmann L., Lowy D. R., Schiller J. T. 1993; Efficient self-assembly of human papillomavirus type 16 L1 and L1–L2 into virus-like particles. J Virol 67:6929–6936
    [Google Scholar]
  12. Li M., Cripe T. P., Estes P. A., Lyon M. K., Rose R. C., Garcea R. L. 1997; Expression of the human papillomavirus type 11 L1 capsid protein in Escherichia coli : characterization of protein domains involved in DNA binding and capsid assembly. J Virol 71:2988–2995
    [Google Scholar]
  13. Liu W. J., Gissmann L., Sun X. Y., Kanjanahaluethai A., Muller M., Doorbar J., Zhou J. 1997; Sequence close to the N-terminus of L2 protein is displayed on the surface of bovine papillomavirus type 1 virions. Virology 227:474–483
    [Google Scholar]
  14. McMillan N. A., Payne E., Frazer I. H., Evander M. 1999; Expression of alpha 6 integrin confers papillomavirus binding upon receptor-negative B-cell. Virology 261:271–274
    [Google Scholar]
  15. Mislick K. A., Baldeschweiler J. D. 1996; Evidence for the role of proteoglycans in cation-mediated gene transfer. Proc Natl Acad Sci US A 93:12349–12354
    [Google Scholar]
  16. Mounkes L. C., Zhong W., Cipres-Palacin G., Heath T. D., Debs R. B. 1999; Proteoglycans mediate cationic liposome–DNA complexes-based gene delivery in vitro and in vivo . J Biol Chem 273:26164–26170
    [Google Scholar]
  17. Müller M., Gissmann L., Cristiano R. J., Sun X. Y., Frazer I. H., Jenson A. B., Alonso A., Zentgraf H., Zhou J. 1995; Papillomavirus binding and uptake by cells from different tissues and species. J Virol 69:948–954
    [Google Scholar]
  18. Roden R. B., Greenstone H. L., Kirnbauer R., Booy F. P., Jessie J., Lowy D., Schiller J. T. 1996; In vitro generation and type-specific neutralization of a human papillomavirus type 16 virion pseudotype. J Virol 70:5875–5883
    [Google Scholar]
  19. Roden R. B. S., Day P. M., Bronzo B. K., Yutzy W. H., IV Yang Y., Lowy D. R., Schiller J. T. 2001; Positively charged termini of the L2 minor capsid protein are necessary for papillomavirus infection. J Virol 75:10493–10497
    [Google Scholar]
  20. Rossi J. L., Gissmann L., Jansen K., Müller M. 2000; Assembly of human papillomavirus type 16 in Saccharomyces cerevisiae . Hum Gene Ther 20:1165–1176
    [Google Scholar]
  21. Schäfer F., Florin L., Sapp M. 2002; DNA binding of L1 is required for human papillomavirus morphogenesis in vivo. Virology 295:172–181
    [Google Scholar]
  22. Schiller J. T., Roden R. B. S. 1995; Papillomavirus-like particles. Papillomavirus Rep 6:121–128
    [Google Scholar]
  23. Stauffer Y., Raj K., Masternak K., Beard P. 1998; Infectious human papillomavirus type 18 pseudovirions. J Mol Biol 283:529–536
    [Google Scholar]
  24. Sun X. Y., Frazer I. H., Müller M., Gissmann L., Zhou J. 1995; Sequences required for the nuclear targeting and accumulation of human papillomavirus type 6B L2 protein. Virology 213:321–327
    [Google Scholar]
  25. Touzé A., Coursaget P. 1998; In vitro gene transfer using human papillomavirus-like particles. Nucleic Acids Res 26:1317–1323
    [Google Scholar]
  26. Touzé A., Mahé D., El Mehdaoui S., Combita A. L., Bousarghin L., Coursaget P. 2000; The 9 C-terminal amino acids of the major capsid protein of the human papillomavirus type 16 are essential for DNA binding and gene transfer capacity. FEMS Microbiol Lett 189:121–127
    [Google Scholar]
  27. Touzé A., Bousarghin L., Ster C., Combita A.-L., Roingeard P., Coursaget P. 2001; Gene transfer using human polyomavirus BK virus-like particles expressed in insect cells. J Gen Virol 82:3005–3009
    [Google Scholar]
  28. Unckell F., Streeck R. E., Sapp M. 1997; Generation of pseudovirions of human papillomavirus type 33. J Virol 71:2934–2939
    [Google Scholar]
  29. Zhao K. N., Frazer I. H., Jun Liu W., Williams M., Zhou J. 1999; Nucleotides 1506–1625 of bovine papillomavirus type 1 genome can enhance DNA packaging by L1/L2 capsids. Virology 259:211–218
    [Google Scholar]
  30. Zhou J., Doorbar J., Sun X. Y., Crawford L. V., McLean C. S., Frazer I. H. 1991; Identification of the nuclear localization signal of human papillomavirus type 16 L1 protein. Virology 185:625–632
    [Google Scholar]
  31. Zhou J., Sun X. Y., Louis K., Frazer I. H. 1994; Interaction of human papillomavirus (HPV) type 16 capsid proteins with HPV DNA requires an intact L2 N-terminal sequence. J Virol 68:619–625
    [Google Scholar]
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