Gene expression analysis of murine cells producing amphotropic mouse leukaemia virus at a cultivation temperature of 32 and 37 °C
Beer, Christiane and Buhr, Petra and Hahn, Heidi and Laubner, Daniela and Wirth, Manfred,, 84, 1677-1686 (2003),
doi = https://doi.org/10.1099/vir.0.18871-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Cultivation of retrovirus packaging cells at 32 °C represents a common procedure to achieve high titres in mouse retrovirus production. Gene expression profiling of mouse NIH 3T3 cells producing amphotropic mouse leukaemia virus 4070A revealed that 10 % of the 1176 cellular genes investigated were regulated by temperature shift (37/32 °C), while 5 % were affected by retrovirus infection. Strikingly, retrovirus production at 32 °C activated the cholesterol biosynthesis/transport pathway and caused an increase in plasma membrane cholesterol levels. Furthermore, these conditions resulted in transcriptional activation of smoothened (smo), patched (ptc) and gli-1; Smo, Ptc and Gli-1, as well as cholesterol, are components of the Sonic hedgehog (Shh) signalling pathway, which directs pattern formation, diversification and tumourigenesis in mammalian cells. These findings suggest a link between cultivation at 32 °C, production of MLV-A and the Shh signalling pathway.,
language=,
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