@article{mbs:/content/journal/jgv/10.1099/vir.0.18998-0, author = "Felipe, Pablo de and Izquierdo, Marta", title = "Construction and characterization of pentacistronic retrovirus vectors", journal= "Journal of General Virology", year = "2003", volume = "84", number = "5", pages = "1281-1285", doi = "https://doi.org/10.1099/vir.0.18998-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.18998-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The picornavirus foot-and-mouth disease virus 2A sequence was combined with three different internal ribosome entry segments to construct and characterize three independent pentacistronic retroviruses of different sizes. Efficient co-expression of the five proteins was successful and titres obtained for these pentacistronic virus vectors (final genome size ∼7·9 kb) were comparable to those of vector systems with shorter genomes. Other vectors constructed that exceeded the genome length of the wild-type virus suffered frequent deletions.", }