Construction and characterization of pentacistronic retrovirus vectors
Felipe, Pablo de and Izquierdo, Marta,, 84, 1281-1285 (2003),
doi = https://doi.org/10.1099/vir.0.18998-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= The picornavirus foot-and-mouth disease virus 2A sequence was combined with three different internal ribosome entry segments to construct and characterize three independent pentacistronic retroviruses of different sizes. Efficient co-expression of the five proteins was successful and titres obtained for these pentacistronic virus vectors (final genome size ∼7·9 kb) were comparable to those of vector systems with shorter genomes. Other vectors constructed that exceeded the genome length of the wild-type virus suffered frequent deletions.,
language=,
type=