RT Journal Article SR Electronic(1) A1 Felipe, Pablo de A1 Izquierdo, MartaYR 2003 T1 Construction and characterization of pentacistronic retrovirus vectors JF Journal of General Virology, VO 84 IS 5 SP 1281 OP 1285 DO https://doi.org/10.1099/vir.0.18998-0 PB Microbiology Society, SN 1465-2099, AB The picornavirus foot-and-mouth disease virus 2A sequence was combined with three different internal ribosome entry segments to construct and characterize three independent pentacistronic retroviruses of different sizes. Efficient co-expression of the five proteins was successful and titres obtained for these pentacistronic virus vectors (final genome size ∼7·9 kb) were comparable to those of vector systems with shorter genomes. Other vectors constructed that exceeded the genome length of the wild-type virus suffered frequent deletions., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.18998-0