1887

Journal of General Virology header logo

Volume 84, Issue 8

Subgroup C avian metapneumovirus (MPV) and the recently isolated human MPV exhibit a common organization but have extensive sequence divergence in their putative SH and G genes Free

Abstract

The genes encoding the putative small hydrophobic (SH), attachment (G) and polymerase (L) proteins of the Colorado isolate of subgroup C avian pneumovirus (APV) were entirely or partially sequenced. They all included metapneumovirus (MPV)-like gene start and gene end sequences. The deduced Colorado SH protein shared 26·9 and 21·7 % aa identity with its counterpart in human MPV (hMPV) and APV subgroup A, respectively, but its only significant aa similarities were to hMPV. Conserved features included a common hydrophobicity profile with an unique transmembrane domain and the conservation of most extracellular cysteine residues. The Colorado putative G gene encoded several ORFs, the longer of which encoded a 252 aa long type II glycoprotein with aa similarities to hMPV G only (20·6 % overall aa identity with seven conserved N-terminal residues). The putative Colorado G protein shared, at best, 21·0 % aa identity with its counterparts in the other APV subgroups and did not contain the extracellular cysteine residues and short aa stretch highly conserved in other APVs. The N-terminal end of the Colorado L protein exhibited 73·6 and 54·9 % aa identity with hMPV and APV subgroup A, respectively, with four aa blocks highly conserved among . Phylogenetic analysis performed on the nt sequences confirmed that the L sequences from MPVs were genetically related, whereas analysis of the G sequences revealed that among MPVs, only APV subgroups A, B and D clustered together, independently of both the Colorado isolate and hMPV, which shared weak genetic relatedness at the G gene level.

  • Received:
  • Accepted:
  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19043-0
2003-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/8/vir842169.html?itemId=/content/journal/jgv/10.1099/vir.0.19043-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
     [Google Scholar]
  2. Bäyon-Auboyer M. H., Jestin V., Toquin D., Cherbonnel M., Eterradossi N. 1999; Comparison of F-, G- and N-based RT-PCR protocols with conventional virological procedures for the detection and typing of turkey rhinotracheitis virus. Arch Virol 144:1091–1109
     [Google Scholar]
  3. Bäyon-Auboyer M. H., Arnauld C., Toquin D., Eterradossi N. 2000; Nucleotide sequences of the F, L and G protein genes of two non-A/non-B avian pneumoviruses (APV) reveal a novel APV subgroup. J Gen Virol 81:2723–2733
     [Google Scholar]
  4. Bell I. G., Alexander D. J. 1990; Failure to detect antibody to turkey rhinotracheitis virus in Australian poultry flocks. Aust Vet J 67:232–233
     [Google Scholar]
  5. Bukreyev A., Whitehead S. S., Murphy B. R., Collins P. L. 1997; Recombinant respiratory syncytial virus from which the entire SH gene has been deleted grows efficiently in cell culture and exhibits site-specific attenuation in the respiratory tract of the mouse. J Virol 71:8973–8982
     [Google Scholar]
  6. Buys S. B., Du Preez J. H. 1980; A preliminary report on the isolation of a virus causing sinusitis in turkey in South Africa and attempts to attenuate the virus. Turkeys 28:36–46
     [Google Scholar]
  7. Chen M. D., Vasquez M., Kahn J. S., Buonocore L. 2000; Conservation of the respiratory syncytial virus SH gene. J Infect Dis 182:1228–1233
     [Google Scholar]
  8. Claros M. G., von Heijne G. 1994; TopPredII: an improved software for membrane protein structure predictions. Comput Appl Biosci 10:685–686
     [Google Scholar]
  9. Collins M. S., Gough R. E., Alexander D. J. 1993; Antigenic differentiation of avian pneumovirus isolates using polyclonal antisera and mouse monoclonal antibodies. Avian Pathol 22:469–479
     [Google Scholar]
  10. Cook J. K. 2000; Avian rhinotracheitis. Rev Sci Tech 19:602–613
     [Google Scholar]
  11. Cook J. K. A., Jones B. V., Ellis M. M., LI J., Cavanagh D. 1993; Antigenic differentiation of strains of turkey rhinotracheitis virus using monoclonal antibodies. Avian Pathol 22:257–273
     [Google Scholar]
  12. Cook J. K. A., Huggins M. B., Woods M. A., Orbell S. J., Mockett A. P. A. 1995; Protection provided by a commercially available vaccine against different strains of turkey rhinotracheitis virus. Vet Rec 136:392–393
     [Google Scholar]
  13. Cook J. K. A., Huggins M. B., Orbell S. J., Senne D. A. 1999; Preliminary antigenic characterization of an avian pneumovirus isolated from commercial turkeys in Colorado, USA. Avian Pathol 28:607–617
     [Google Scholar]
  14. Eterradossi N., Toquin D., Guittet M., Bennejean G. 1995; Evaluation of different turkey rhinotracheitis viruses used as antigens for serological testing following live vaccination and challenge. Zentralbl Veterinarmed B 342:175–186
     [Google Scholar]
  15. Felsenstein J. 1993 phylip: Phylogenic Inference Package, version 3.52c Department of Genetics, University of Washington; Seattle, WA, USA:
     [Google Scholar]
  16. Freymuth F., Eterradossi N., Toquin D., Jestin V., Vabret A., Petitjean J., Gouarin S. 2002; First detection of human metapneumovirus in hospitalized children in France. Virologie 6:S14 (in French
    [Google Scholar]
  17. Giraud P., Bennejean G., Guittet M., Toquin D. 1986; Turkey rhinotracheitis in France: preliminary investigations on a ciliostatic virus. Vet Rec 119:606–607
     [Google Scholar]
  18. Gupta R., Jung E., Brunak S. 2002; Prediction of N-glycosylation sites in human proteins.
  19. Hansen J. E., Lund O., Tolstrup N., Gooley A. A., Williams K. L., Brunak S. 1998; NetOglyc: prediction of mucin type O -glycosylation sites based on sequence context and surface accessibility. Glycoconj J 15:115–130
     [Google Scholar]
  20. Hendricks D. A., Baradaran K., McIntosh K., Patterson J. L. 1987; Appearance of a soluble form of the G protein of respiratory syncytial virus in fluids of infected cells. J Gen Virol 68:1705–1714
     [Google Scholar]
  21. Juhasz K., Easton A. J. 1994; Extensive sequence variation in the attachment (G) protein gene of avian pneumovirus: evidence for two distinct subgroups. J Gen Virol 75:2873–2880
     [Google Scholar]
  22. Karger A., Schmidt U., Buchholz U. J. 2001; Recombinant bovine respiratory syncytial virus with deletions of the G or SH genes: G and F proteins bind heparin. J Gen Virol 82:631–640
     [Google Scholar]
  23. Karron R. A., Buonagurio D. A., Georgiu A. F. 8 other authors 1997; Respiratory syncytial virus (RSV) SH and G proteins are not essential for viral replication in vitro : clinical evaluation and molecular characterization of a cold-passaged, attenuated RSV subgroup B mutant. Proc Natl Acad Sci U S A 94:13961–13966
     [Google Scholar]
  24. Lichtenstein D. L., Roberts S. R., Wertz G. W., Ball L. A. 1996; Definition and functional analysis of the signal anchor domain of the human respiratory syncytial virus glycoprotein G. J Gen Virol 77:109–118
     [Google Scholar]
  25. Ling R., Easton A. J., Pringle C. R. 1992; Sequence analysis of the 22K, SH and G genes of turkey rhinotracheitis virus and their intergenic regions reveals a gene order different from that of other pneumoviruses. J Gen Virol 73:1709–1715
     [Google Scholar]
  26. McDougall J. S., Cook J. K. A. 1986; Turkey rhinotracheitis: preliminary investigations. Vet Rec 118:206–207
     [Google Scholar]
  27. Nielsen H., Engelbrecht J., Brunak S., von Heijne G. 1997; Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 10:1–6
     [Google Scholar]
  28. Nissen M. D., Sieberg D. J., Mackay I. M., Sloots TP., Withers S. J. 2002; Evidence of human metapneumovirus in Australian children. Med J Aust 176:188
     [Google Scholar]
  29. Peret T. C., Boivin G., Li Y., Couillard M., Humphrey C., Osterhaus A. D., Erdman D. D., Anderson L. J. 2002; Characterization of human metapneumoviruses isolated from patients in North America. J Infect Dis 185:1660–1663
     [Google Scholar]
  30. Pringle C. R. 1998; Virus taxonomy – San Diego 1998. Arch Virol 143:1449–1459
     [Google Scholar]
  31. Randhawa J. S., Wilson S. D., Tolley K. P., Cavanagh D., Pringle C. R., Easton A. J. 1996; Nucleotide sequence of the gene encoding the viral polymerase of avian pneumovirus. J Gen Virol 77:3047–3051
     [Google Scholar]
  32. Roberts S. R., Lichtenstein D. L., Ball L. A., Wertz G. W. 1994; The membrane-associated and secreted forms of the respiratory syncytial virus attachment glycoprotein G are synthesized from alternative initiation codons. J Virol 68:4538–4546
     [Google Scholar]
  33. Rueda P., Delgado T., Portela A., Melero J. A., Garcia-Barreno B. 1991; Premature stop codons in the G glycoprotein of human respiratory syncytial viruses resistant to neutralization by monoclonal antibodies. J Virol 65:3374–3378
     [Google Scholar]
  34. Seal B. S. 1998; Matrix protein gene nucleotide and predicted amino acid sequence demonstrate that the first US avian pneumovirus isolate is distinct from European strains. Virus Res 58:45–52
     [Google Scholar]
  35. Seal B. S. 2000; Avian pneumoviruses and emergence of a new type in the United States of America. Anim Health Res Rev 1:67–72
     [Google Scholar]
  36. Senne D. A., Edson R. K., Pedersen J. C., Panigraphy B. 1997; Avian pneumovirus update. In Proceedings of the 134th Annual Congress of the American Veterinary Medical Association p–190 Reno, NV, USA:
     [Google Scholar]
  37. Shin H. J., Cameron K. T., Jacobs J. A. 8 other authors 2002; Molecular epidemiology of subgroup C avian pneumoviruses isolated in the United States and comparison with subgroup A and B viruses. J Clin Microbiol 40:1687–1693
     [Google Scholar]
  38. Stec D. S., Hill M. G. III, Collins P. L. 1991; Sequence analysis of the polymerase L gene of human respiratory syncytial virus and predicted phylogeny of nonsegmented negative-strand viruses. Virology 183:273–287
     [Google Scholar]
  39. Techaarpornkul S., Barretto N., Peeples M. E. 2001; Functional analysis of recombinant respiratory syncytial virus deletion mutants lacking the small hydrophobic and/or attachment glycoprotein gene. J Virol 75:6825–6834
     [Google Scholar]
  40. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
     [Google Scholar]
  41. Toquin D., Eterradossi N., Guittet M., Bennejean G. 1992; Infectious rhinotracheitis in turkeys: antigenic differences revealed by ELISA. In European Commission Proceedings New and Evolving Pathogens Virus Diseases of Poultry pp  111–121 Edited by McNulty M. S., McFerran J. B. Publication VI/4104/94-EN: III-121
    [Google Scholar]
  42. Toquin D., Eterradossi N., Guittet M. 1996; Use of a related ELISA antigen for efficient TRT serological testing following live vaccination. Vet Rec 139:71–72
     [Google Scholar]
  43. Toquin D., Bäyon-Auboyer M. H., Jestin V., Eterradossi N., Morin H. 1999a; Isolation of a pneumovirus from a Muscovy duck. Vet Rec 145:680
     [Google Scholar]
  44. Toquin D., Bäyon-Auboyer M. H., Jestin V., Eterradossi N. 1999b; Serological response and cross protection following infection by a non-A, non-B strain of turkey infectious rhinotracheitis virus. . . Proceedings Troisièmes Journées de la Recherche Avicole , pp. 221–224. (St Malo, France: 23–25 March 1999 (in French
    [Google Scholar]
  45. Toquin D., Bäyon-Auboyer M. H., Senne D. A., Eterradossi N. 2000; Lack of antigenic relationship between French and recent North American non-A/non-B turkey rhinotracheitis viruses. Avian Dis 44:977–982
     [Google Scholar]
  46. van den Hoogen B. G., de Jong J. C., Groen J., Kuiken T., de Groot R., Fouchier R. A., Osterhaus A. D. 2001; A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med 7:719–724
     [Google Scholar]
  47. van den Hoogen B. G., Besteboer T. M., Osterhaus A. D., Fouchier R. A. 2002; Analysis of the genomic sequence of a human metapneumovirus. Virology 295:119–132
     [Google Scholar]
  48. Yu Q., Davis P. J., Li J., Cavanagh D. 1992; Cloning and sequencing of the matrix protein (M) gene of turkey rhinotracheitis virus reveal a gene order different from that of respiratory syncytial virus. Virology 186:426–434
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19043-0
Loading
Subgroup C avian metapneumovirus (MPV) and the recently isolated human MPV exhibit a common organization but have extensive sequence divergence in their putative SH and G genes
J. Gen. Virol. 84, 2169 (2003); https://doi.org/10.1099/vir.0.19043-0
/content/journal/jgv/10.1099/vir.0.19043-0
/content/journal/jgv/10.1099/vir.0.19043-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error