1887

Abstract

A DNA vaccination regime was investigated previously in rhesus macaques using a full-genome human immunodeficiency virus type 1 (HIV-1) plasmid, which, due to mutations in the nucleocapsid (NC) proteins, produced only non-infectious HIV-1 particles ( Akahata ., , 116–124, 2000 ). In that study, four monkeys were injected intramuscularly 14 times with the plasmid. All of them showed immunological responses against HIV-1 and partial protection from challenge with a simian immunodeficiency virus/HIV (SHIV) chimeric virus. To improve this DNA vaccination regime, the plasmid used for vaccination was changed. In the present study, four macaques were injected intramuscularly eight times with a full-genome SHIV plasmid that produces non-infectious SHIV particles. CTL activities were higher than those observed in monkeys vaccinated previously with the HIV-1 plasmid. In all macaques vaccinated, peak plasma virus loads after homologous challenge with SHIV were two to three orders of magnitude lower than those of the naive controls, and virus loads fell below the level of detection at 6 weeks post-challenge. This suggested that the vaccination regime in this study was partially effective and better than the previous regime.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19082-0
2003-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/8/vir842237.html?itemId=/content/journal/jgv/10.1099/vir.0.19082-0&mimeType=html&fmt=ahah

References

  1. Akahata W., Ido E., Shimada T. 7 other authors 2000; DNA vaccination of macaques by a full genome HIV-1 plasmid which produces noninfectious virus particles. Virology 275:116–124
    [Google Scholar]
  2. Alter H. J., Eichberg J. W., Masur H., Saxinger W. C., Gallo R., Macher A. M., Lane H. C., Fauci A. S. 1984; Transmission of HTLV-III infection from human plasma to chimpanzees: an animal model for AIDS. Science 226:549–552
    [Google Scholar]
  3. Baba T. W., Liska V., Khimani A. H. 8 other authors 1999; Live attenuated, multiply deleted simian immunodeficiency virus causes AIDS in infant and adult macaques. Nat Med 5:194–203
    [Google Scholar]
  4. Barouch D. H., Santra S., Schmitz J. E. 26 other authors 2000; Control of viremia and prevention of clinical AIDS in rhesus monkeys by cytokine-augmented DNA vaccination. Science 290:486–492
    [Google Scholar]
  5. Boyer J. D., Ugen K. E., Wang B. 12 other authors 1997; Protection of chimpanzees from high-dose heterologous HIV-1 challenge by DNA vaccination. Nat Med 3:526–532
    [Google Scholar]
  6. Daniel M. D., Kirchhoff F., Czajak S. C., Sehgal P. K., Desrosiers R. C. 1992; Protective effects of a live attenuated SIV vaccine with a deletion in the nef gene. Science 258:1938–1941
    [Google Scholar]
  7. Fuller D. H., Haynes J. R. 1994; A qualitative progression in HIV type 1 glycoprotein 120-specific cytotoxic cellular and humoral immune responses in mice receiving a DNA-based glycoprotein 120 vaccine. AIDS Res Hum Retroviruses 10:1433–1441
    [Google Scholar]
  8. Gorelick R. J., Nigida S. M. Jr, Bess J. W. Jr, Arthur L. O., Henderson L. E., Rein A. 1990; Noninfectious human immunodeficiency virus type 1 mutants deficient in genomic RNA. J Virol 64:3207–3211
    [Google Scholar]
  9. Gorelick R. J., Benveniste R. E., Lifson J. D. 10 other authors 2000; Protection of Macaca nemestrina from disease following pathogenic simian immunodeficiency virus (SIV) challenge: utilization of SIV nucleocapsid mutant DNA vaccines with and without an SIV protein boost. J Virol 74:11935–11949
    [Google Scholar]
  10. Hanke T., Samuel R. V., Blanchard T. J. 9 other authors 1999; Effective induction of simian immunodeficiency virus-specific cytotoxic T lymphocytes in macaques by using a multiepitope gene and DNA prime-modified vaccinia virus Ankara boost vaccination regimen. J Virol 73:7524–7532
    [Google Scholar]
  11. Haynes J. R., Fuller D. H., Eisenbraun M. D., Ford M. J., Pertmer T. M. 1994; Accell particle-mediated DNA immunization elicits humoral, cytotoxic, and protective immune responses. AIDS Res Hum Retroviruses 10:S43–S45
    [Google Scholar]
  12. Hosie M. J., Flynn J. N., Rigby M. A. 9 other authors 1998; DNA vaccination affords significant protection against feline immunodeficiency virus infection without inducing detectable antiviral antibodies. J Virol 72:7310–7319
    [Google Scholar]
  13. Kestler H., Kodama T., Ringler D. other authors 1990; Induction of AIDS in rhesus monkeys by molecularly cloned simian immunodeficiency virus. Science 248:1109–1112
    [Google Scholar]
  14. Kuwata T., Igarashi T., Ido E., Jin M., Mizuno A., Chen J., Hayami M. 1995; Construction of human immunodeficiency virus 1/simian immunodeficiency virus strain mac chimeric viruses having vpr and/or nef of different parental origins and their in vitro and in vivo replication. J Gen Virol 76:2181–2191
    [Google Scholar]
  15. Letvin N. L., Montefiori D. C., Yasutomi Y. 9 other authors 1997; Potent, protective anti-HIV immune responses generated by bimodal HIV envelope DNA plus protein vaccination. Proc Natl Acad Sci U S A 94:9378–9383
    [Google Scholar]
  16. Meric C., Goff S. P. 1989; Characterization of Moloney murine leukemia virus mutants with single-amino-acid substitutions in the Cys-His box of the nucleocapsid protein. J Virol 63:1558–1568
    [Google Scholar]
  17. Mizuno A., Ido E., Goto T., Kuwata T., Nakai M., Hayami M. 1996; Mutational analysis of two zinc-finger motifs in HIV type 1 nucleocapsid proteins: effects on proteolytic processing of Gag precursors and particle formation. AIDS Res Hum Retroviruses 12:793–800
    [Google Scholar]
  18. Pachl C., Todd J. A., Kern D. G. other authors 1995; Rapid and precise quantification of HIV-1 RNA in plasma using a branched DNA signal amplification assay. J Acquir Immune Defic Syndr Hum Retrovirol 8:446–454
    [Google Scholar]
  19. Robinson H. L., Montefiori D. C., Johnson R. P. 14 other authors 1999; Neutralizing antibody-independent containment of immunodeficiency virus challenges by DNA priming and recombinant pox virus booster immunizations. Nat Med 5:526–534
    [Google Scholar]
  20. Shibata R., Kawamura M., Sakai H., Hayami M., Ishimoto A., Adachi A. 1991; Generation of a chimeric human and simian immunodeficiency virus infectious to monkey peripheral blood mononuclear cells. J Virol 65:3514–3520
    [Google Scholar]
  21. Shibata R., Siemon C., Czajak S. C., Desrosiers R. C., Martin M. A. 1997; Live, attenuated simian immunodeficiency virus vaccines elicit potent resistance against a challenge with a human immunodeficiency virus type 1 chimeric virus. J Virol 71:8141–8148
    [Google Scholar]
  22. Suryanarayana K., Wiltrout T. A., Vasquez G. M., Hirsch V. M., Lifson J. D. 1998; Plasma SIV RNA viral load determination by real-time quantification of product generation in reverse transcriptase-polymerase chain reaction. AIDS Res Hum Retroviruses 14:183–189
    [Google Scholar]
  23. Tsai C. C., Follis K. E., Beck T. W., Sabo A., Bischofberger N., Dailey P. J. 1997; Effects of r-9-(2-phosphonylmethoxypropyl)adenine monotherapy on chronic SIV infection in macaques. AIDS Res Hum Retroviruses 13:707–712
    [Google Scholar]
  24. Ui M., Kuwata T., Igarashi T. 9 other authors 1999; Protection of macaques against a SHIV with a homologous HIV-1 Env and a pathogenic SHIV-89.6P with a heterologous Env by vaccination with multiple gene-deleted SHIVs. Virology 265:252–263
    [Google Scholar]
  25. Yamamoto H., Miller M. D., Watkins D. I., Snyder G. B., Chase N. E., Mazzara G. P., Gritz L., Panicali D. L., Letvin N. L. 1990; Two distinct lymphocyte populations mediate simian immunodeficiency virus envelope-specific target cell lysis. J Immunol 145:3740–3746
    [Google Scholar]
  26. Yasutomi Y., Koenig S., Woods R. M. other authors 1995; A vaccine-elicited, single viral epitope-specific cytotoxic T lymphocyte response does not protect against intravenous, cell-free simian immunodeficiency virus challenge. J Virol 69:2279–2284
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19082-0
Loading
/content/journal/jgv/10.1099/vir.0.19082-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error