@article{mbs:/content/journal/jgv/10.1099/vir.0.19083-0, author = "Titterington, Jane S. and Nun, Tamara K. and Passarelli, A. Lorena", title = "Functional dissection of the baculovirus late expression factor-8 gene: sequence requirements for late gene promoter activation", journal= "Journal of General Virology", year = "2003", volume = "84", number = "7", pages = "1817-1826", doi = "https://doi.org/10.1099/vir.0.19083-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19083-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The late expression factor-8 gene (lef-8) of Autographa californica M nucleopolyhedrovirus encodes the largest subunit of the virally encoded DNA-directed RNA polymerase specific for the transcription of late and very late viral genes. The sequence of lef-8 predicts a C-terminal motif of 13 amino acids that is conserved in other polymerases. Detailed mutagenesis throughout lef-8 was performed, including this C-terminal motif, to define sequences required for late promoter activation. It was found that the conserved C-terminal motif was critical for late gene expression. In addition, regions throughout the entire lef-8-encoding sequence were important for optimal function, suggesting complex protein–protein and protein–DNA interrelationships in the late gene-specific viral transcriptosome.", }