1887

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Volume 84, Issue 7

Antibody-mediated growth of infectious salmon anaemia virus in macrophage-like fish cell lines Free

Abstract

Infectious salmon anaemia virus (ISAV), a pathogen in marine aquaculture, belongs to the genus , family . There is limited information on how ISAV interacts with host defences. To study ISAV–antibody interactions, virus neutralization (VN) assays were performed in the cell lines CHSE-214, SHK-1 and TO using three strains of ISAV and rabbit or fish anti-ISAV sera. Homologous VN titres of >1 : 1280 in CHSE-214 cells corresponded to titres of only 1 : 80 in the macrophage-like fish cell lines SHK-1 and TO, despite using 1000 and 2000 times less virus, respectively. However, rabbit antiserum to infectious pancreatic necrosis virus (IPNV) had a VN titre of 1 : 10 260 against IPNV in both CHSE-214 and TO cells. Poor ISAV neutralization in TO cells was attributed to Fc receptors mediating virus infectivity, because (1) neutralization by rabbit antiserum to ISAV was increased 48-fold in the presence of staphylococcal Protein A and (2) when using FITC-labelled virus and spectrofluorometry, a significant increase (=0·018) in the intensity of fluorescence of intracellular virus was observed in assays of virus–antiserum mixtures in the absence of Protein A as compared to those in the presence of Protein A. Neutralization of ISAV with fish antisera was observed only in CHSE-214 cells, as Protein A could not restore neutralization in TO cells. These findings demonstrate for the first time antibody-mediated infection of macrophage-like fish cell lines by a fish virus, ISAV, and, as ISAV in Atlantic salmon targets leukocytic and endothelial cells, this may have implications for ISA pathogenesis and vaccination.

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2003-07-01
2024-04-18
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Antibody-mediated growth of infectious salmon anaemia virus in macrophage-like fish cell lines
J. Gen. Virol. 84, 1701 (2003); https://doi.org/10.1099/vir.0.19087-0
/content/journal/jgv/10.1099/vir.0.19087-0
/content/journal/jgv/10.1099/vir.0.19087-0
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