1887

Abstract

The nucleocapsid protein (NP) of Newcastle disease virus expressed in assembled as ring- and herringbone-like particles. In order to identify the contiguous NP sequence essential for assembly of these particles, 11 N- or C-terminally deleted NP mutants were constructed and their ability to self-assemble was tested. The results indicate that a large part of the NP N-terminal end, encompassing amino acids 1 to 375, is required for proper folding to form a herringbone-like structure. In contrast, the C-terminal end covering amino acids 376 to 489 was dispensable for the formation of herringbone-like particles. A region located between amino acids 375 to 439 may play a role in regulating the length of the herringbone-like particles. Mutants with amino acid deletions further from the C-terminal end (84, 98, 109 and 114 amino acids) tended to form longer particles compared to mutants with shorter deletions (25 and 49 amino acids).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19107-0
2003-08-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/8/vir842163.html?itemId=/content/journal/jgv/10.1099/vir.0.19107-0&mimeType=html&fmt=ahah

References

  1. Alexander D. J. 1988; Newcastle disease virus – an avian paramyxovirus structure. In Newcastle Disease pp  11–22 Edited by Alexander D. J. Boston: Kluwer Academic Publishers;
    [Google Scholar]
  2. Bankamp B., Horikami S. M., Thompson P. D., Huber M., Billeter M., Moyer S. A. 1996; Domains of the measles virus N protein required for binding to P protein and self-assembly. Virology 216:272–277
    [Google Scholar]
  3. Bhella D., Raiph A., Murphy L. B., Yeo R. P. 2002; Significant differences in nucleocapsid morphology within the Paramyxoviridae . J Gen Virol 83:1931–1939
    [Google Scholar]
  4. Buchholz C. J., Spehner D., Drillien R., Neubert W. J., Homann H. E. 1993; The conserved N-terminal region of Sendai virus nucleocapsid protein NP is required for nucleocapsid assembly. J Virol 67:5803–5812
    [Google Scholar]
  5. Buchholz C. J., Retzler C., Homann H. E., Neubert W. J. 1994; The carboxy-terminal domain of Sendai virus nucleocapsid protein is involved in complex formation between phosphoprotein and nucleocapsid-like particles. Virology 204:770–776
    [Google Scholar]
  6. Curran J., Homann H., Buchholz C., Rochat S., Neubert W., Kolakofsky D. 1993; The hypervariable C-terminal tail of the Sendai paramyxovirus nucleocapsid protein is required for template function but not for RNA encapsidation. J Virol 67:4358–4364
    [Google Scholar]
  7. Egelman E. H., Wu S. S., Amrein M., Portner A., Murti G. 1989; The Sendai virus nucleocapsid exists in at least four different helical states. J Virol 63:2233–2243
    [Google Scholar]
  8. Elton D., Medcalf E., Bishop K., Digard P. 1999; Oligomerization of the influenza virus nucleoprotein: identification of positive and negative sequence elements. Virology 260:190–200
    [Google Scholar]
  9. Errington W., Emmerson P. T. 1997; Assembly of recombinant Newcastle disease virus nucleocapsid protein into nucleocapsid-like structures is inhibited by the phosphoprotein. J Gen Virol 78:2335–2339
    [Google Scholar]
  10. Fooks A. R., Stephenson J. R., Warnes A., Dowsett A. B., Rima B. K., Wilkinson G. W. G. 1993; Measles virus nucleocapsid protein expressed in insect cells assembles into nucleocapsid-like structures. J Gen Virol 74:1439–1444
    [Google Scholar]
  11. Horikami S. M., Curran J., Kolakofsky D., Moyer S. A. 1992; Complexes of Sendai virus NP-P and P-L proteins are required for defective interfering particle genome replication in vitro . J Virol 66:4901–4908
    [Google Scholar]
  12. Kho C. L., Tan W. S., Yusoff K. 2001a; Production of the nucleocapsid protein of Newcastle disease virus in Escherichia coli and its assembly into ring- and nucleocapsid-like particles. J Microbiol 39:293–299
    [Google Scholar]
  13. Kho C. L., Tan W. S., Yusoff K. 2001b; Sequence analysis of the nucleocapsid of a Newcastle disease virus heat resistant strain: comparison with other members of the Paramyxoviridae . J Biochem Mol Biol Biophys 5:463–471
    [Google Scholar]
  14. Liston P., Batal R., DiFlumeri C., Briedis D. J. 1997; Protein interaction domains of the measles virus nucleocapsid protein (NP. Arch Virol 142:305–321
    [Google Scholar]
  15. Morgan E. M., Re G. G., Kingsbury D. W. 1984; Complete sequence of the Sendai virus NP gene from a cloned insert. Virology 135:279–287
    [Google Scholar]
  16. Mountcastle W. E., Compans R. W., Lackland H., Choppin P. W. 1974; Proteolytic cleavage of subunits of simian virus 5. J Virol 14:1253–1261
    [Google Scholar]
  17. Myers T. M., Smallwood S., Moyer S. A. 1999; Identification of nucleocapsid protein residues required for Sendai virus nucleocapsid formation and genome replication. J Gen Virol 80:1383–1391
    [Google Scholar]
  18. Nishio M., Tsurudome M., Ito M., Kawano M., Kusagawa S., Komada H., Ito Y. 1999; Mapping of domains on the human parainfluenza virus type 2 nucleocapsid protein (NP) required for NP–phosphoprotein or NP–NP interaction. J Gen Virol 80:2017–2022
    [Google Scholar]
  19. Phillips R. J., Samson A. C. R., Emmerson P. T. 1998; Nucleotide sequence of the 5′-terminus of Newcastle disease virus and assembly of the complete genomic sequence: agreement with the “rule of six”. Arch Virol 143:1993–2002
    [Google Scholar]
  20. Rabu A., Tan W. S., Kho C. L., Omar A. R., Yusoff K. 2002; Chimeric Newcastle disease virus nucleocapsid with parts of viral hemagglutinin-neuraminidase and fusion proteins. Acta Virol 46:211–217
    [Google Scholar]
  21. Rima B. K., Alexander D. J., Billeter M. A. 7 other authors 1995; The Paramyxoviridae . In Virus Taxonomy, Classification and Nomenclature of Viruses . Sixth Report of the International Committee on Taxonomy of Viruses pp  268–274 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Moya M. A., Summers M. D. Vienna & New York: Springer Verlag;
    [Google Scholar]
  22. Samson A. C. R. 1988; Virus structure. In Newcastle Disease pp  23–44 Edited by Alexander D. J. Boston: Kluwer Academic Publishers;
    [Google Scholar]
  23. Schoehn G., Iseni F., Mavrakis M., Blondel D., Ruigrok R. W. H. 2001; Structure of recombinant rabies virus nucleoprotein-RNA complex and identification of the phosphoprotein binding site. J Virol 75:490–498
    [Google Scholar]
  24. Spehner D., Kirn A., Drillien R. 1991; Assembly of nucleocapsid-like structures in animal cells infected with a vaccinia virus recombinant encoding the measles virus nucleoprotein. J Virol 65:6296–6300
    [Google Scholar]
  25. Sprague J., Condra J. H., Arnheiter H., Lazzarini R. A. 1983; Expression of a recombinant DNA gene coding for the vesicular stomatitis virus nucleocapsid protein. J Virol 45:773–781
    [Google Scholar]
  26. Yusoff K., Tan W. S. 2001; Newcastle disease virus: macromolecules and opportunities. Avian Pathol 30:439–455
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19107-0
Loading
/content/journal/jgv/10.1099/vir.0.19107-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error