RT Journal Article SR Electronic(1) A1 Magiorkinis, Gkikas A1 Paraskevis, Dimitrios A1 Vandamme, Anne-Mieke A1 Magiorkinis, Emmanouil A1 Sypsa, Vana A1 Hatzakis, AngelosYR 2003 T1 In vivo characteristics of human immunodeficiency virus type 1 intersubtype recombination: determination of hot spots and correlation with sequence similarity JF Journal of General Virology, VO 84 IS 10 SP 2715 OP 2722 DO https://doi.org/10.1099/vir.0.19180-0 PB Microbiology Society, SN 1465-2099, AB Recombination plays a pivotal role in the evolutionary process of many different virus species, including retroviruses. Analysis of all human immunodeficiency virus type 1 (HIV-1) intersubtype recombinants revealed that they are more complex than described initially. Recombination frequency is higher within certain genomic regions, such as partial reverse transcriptase (RT), vif/vpr, the first exons of tat/rev, vpu and gp41. A direct correlation was observed between recombination frequency and sequence similarity across the HIV-1 genome, indicating that sufficient sequence similarity is required upstream of the recombination breakpoint. This finding suggests that recombination in vivo may occur preferentially during reverse transcription through the strand displacement-assimilation model rather than the copy-choice model., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19180-0