%0 Journal Article %A Hemmer, Odile %A Dunoyer, Patrice %A Richards, Kenneth %A Fritsch, Christiane %T Mapping of viral RNA sequences required for assembly of peanut clump virus particles %D 2003 %J Journal of General Virology, %V 84 %N 9 %P 2585-2594 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.19247-0 %I Microbiology Society, %X RNA sequences required for assembly into rod-shaped virions of RNA-1 and RNA-2 of Peanut clump virus (PCV) were mapped by testing the ability of different RNA-1 and -2 deletion mutants to be encapsidated in vivo in an RNase-resistant form. Encapsidation of RNA-1 was found to require a sequence domain in the 5′-proximal part of the P15 gene, the 3′-proximal gene of RNA-1. On the other hand, the subgenomic RNA which encodes P15 was not encapsidated, suggesting that other features of RNA-1 are important as well. Two sequences which could drive encapsidation of RNA-2 deletion mutants were located. One was in the 5′-proximal coat protein gene and the other in the P14 gene near the RNA 3′ terminus. There were no obvious sequence homologies between the different assembly initiation sequences. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19247-0