Mapping of viral RNA sequences required for assembly of peanut clump virus particles
Hemmer, Odile and Dunoyer, Patrice and Richards, Kenneth and Fritsch, Christiane,, 84, 2585-2594 (2003),
doi = https://doi.org/10.1099/vir.0.19247-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= RNA sequences required for assembly into rod-shaped virions of RNA-1 and RNA-2 of Peanut clump virus (PCV) were mapped by testing the ability of different RNA-1 and -2 deletion mutants to be encapsidated in vivo in an RNase-resistant form. Encapsidation of RNA-1 was found to require a sequence domain in the 5′-proximal part of the P15 gene, the 3′-proximal gene of RNA-1. On the other hand, the subgenomic RNA which encodes P15 was not encapsidated, suggesting that other features of RNA-1 are important as well. Two sequences which could drive encapsidation of RNA-2 deletion mutants were located. One was in the 5′-proximal coat protein gene and the other in the P14 gene near the RNA 3′ terminus. There were no obvious sequence homologies between the different assembly initiation sequences.,
language=,
type=