1887

Journal of General Virology header logo

Volume 84, Issue 11

Molecular characterization of a novel astrovirus associated with disease in mink Free

Abstract

Pre-weaning diarrhoea is a well-known problem in mink farming in Europe, causing morbidity that varies between farms, regions and season. Different causalities for the disease have been proposed, but only most recently has a novel astrovirus been identified as an important risk factor. In this report, the molecular characterization, origin and evolution of this novel astrovirus of mink are discussed. The polyadenylated, positive-stranded RNA genome was sequenced and found to contain 6610 nt, organized into three ORFs and two short UTRs. A ribosomal frameshift sequence links the 5′ two ORFs, containing sequence motifs for a serine protease (ORF1a) and an RNA-dependent RNA polymerase (ORF1b). The structural proteins are encoded by ORF2 and, presumably, are expressed as a polyprotein precursor to be cleaved into the mature capsid proteins. These results indicate that mink astrovirus (MiAstV) has all of the features typical of members of the . Phylogenetic analyses revealed that MiAstV is distantly related to established astroviruses, showing less than 67 % similarity at the nucleotide level with its closest relative, ovine astrovirus, and even lower identities at the predicted amino acid level. Nevertheless, sequence analysis of MiAstV isolates from geographically distinct Swedish and Danish farms showed much less diversity. This suggests either the spread in the mink population of a virus that has evolved a long time ago or the recent introduction of an ancient virus into a new host species.

  • Received:
  • Accepted:
  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19267-0
2003-11-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/11/vir843087.html?itemId=/content/journal/jgv/10.1099/vir.0.19267-0&mimeType=html&fmt=ahah

References

  1. Aroonprasert D., Fagerland J. A., Kelso N. E., Zheng S., Woode G. N. 1989; Cultivation and partial characterization of bovine astrovirus. Vet Microbiol 19:113–125
     [Google Scholar]
  2. Bass D. M., Qiu S. 2000; Proteolytic processing of the astrovirus capsid. J Virol 74:1810–1814
     [Google Scholar]
  3. Bowden T. R., Westenberg M., Wang L. F., Eaton B. T., Boyle D. B. 2001; Molecular characterization of Menangle virus, a novel paramyxovirus which infects pigs, fruit bats, and humans. Virology 283:358–373
     [Google Scholar]
  4. Dingwall C., Laskey R. A. 1991; Nuclear targeting sequences: a consensus?. Trends Biochem Sci 16:478–481
     [Google Scholar]
  5. Englund L., Chriél M., Dietz H. H., Hedlund K. O. 2002; Astrovirus epidemiologically linked to pre-weaning diarrhoea in mink. Vet Microbiol 85:1–11
     [Google Scholar]
  6. Geigenmüller U., Chew T., Ginzton N., Matsui S. M. 2002a; Processing of nonstructural protein 1a of human astrovirus. J Virol 76:2003–2008
     [Google Scholar]
  7. Geigenmüller U., Ginzton N. H., Matsui S. M. 2002b; Studies on intracellular processing of the capsid protein of human astrovirus serotype 1 in infected cells. J Gen Virol 83:1691–1695
     [Google Scholar]
  8. Gibson C. A., Chen J., Monroe S. A., Denison M. R. 1998; Expression and processing of nonstructural proteins of the human astroviruses. Adv Exp Med Biol 440:387–391
     [Google Scholar]
  9. Gough R. E., Collins M. S., Borland E., Keymer L. F. 1984; Astrovirus-like particles associated with hepatitis in ducklings. Vet Rec 114:279
     [Google Scholar]
  10. Haqshenas G., Shivaprasad H. L., Woolcock P. R., Read D. H., Meng X. J. 2001; Genetic identification and characterization of a novel virus related to human hepatitis E virus from chickens with hepatitis–splenomegaly syndrome in the United States. J Gen Virol 82:2449–2462
     [Google Scholar]
  11. Hoshino Y., Zimmer J. F., Moise N. S., Scott F. W. 1981; Detection of astroviruses in feces of a cat with diarrhea. Arch Virol 70:373–376
     [Google Scholar]
  12. Imada T., Yamaguchi S., Mase M., Tsukamoto K., Kubo M., Morooka A. 2000; Avian nephritis virus (ANV) as a new member of the family Astroviridae and construction of infectious ANV cDNA. J Virol 74:8487–8493
     [Google Scholar]
  13. Jiang B., Monroe S. S., Koonin E. V., Stine S. E., Glass R. I. 1993; RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frameshifting signal that directs the viral replicase synthesis. Proc Natl Acad Sci U S A 90:10539–10543
     [Google Scholar]
  14. Kiang D., Matsui S. M. 2002; Proteolytic processing of a human astrovirus nonstructural protein. J Gen Virol 83:25–34
     [Google Scholar]
  15. Kjeldsberg E., Hem A. 1985; Detection of astroviruses in gut contents of nude and normal mice. Arch Virol 84:135–140
     [Google Scholar]
  16. Koci M. D., Seal B. S., Schultz-Cherry S. 2000; Molecular characterization of an avian astrovirus. J Virol 74:6173–6177
     [Google Scholar]
  17. Krogh A., Larsson B., von Heijne G., Sonnhammer E. L. 2001; Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol 305:567–580
     [Google Scholar]
  18. Lewis T. L., Matsui S. M. 1995; An astrovirus frameshift signal induces ribosomal frameshifting in vitro . Arch Virol 140:1127–1135
     [Google Scholar]
  19. Lewis T. L., Matsui S. M. 1996; Astrovirus ribosomal frameshifting in an infection-transfection transient expression system. J Virol 70:2869–2875
     [Google Scholar]
  20. Lewis T. L., Matsui S. M. 1997; Studies of the astrovirus signal that induces (−1) ribosomal frameshifting. Adv Exp Med Biol 412:323–330
     [Google Scholar]
  21. Lewis T. L., Greenberg H. B., Herrmann J. E., Smith L. S., Matsui S. M. 1994; Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein. J Virol 68:77–83
     [Google Scholar]
  22. Lukashov V. V., Goudsmit J. 2002; Evolutionary relationships among Astroviridae . J Gen Virol 83:1397–1405
     [Google Scholar]
  23. Madeley C. R., Cosgrove B. P. 1975; 28 nm particles in faeces in infantile gastroenteritis. Lancet ii:451–452
     [Google Scholar]
  24. Marczinke B., Bloys A. J., Brown T. D., Willcocks M. M., Carter M. J., Brierley I. 1994; The human astrovirus RNA-dependent RNA polymerase coding region is expressed by ribosomal frameshifting. J Virol 68:5588–5595
     [Google Scholar]
  25. Matsui S. M., Greenberg H. B. 2001; Astroviruses. In Fields Virology , 4th edn. pp  875–893 Edited by Knipe D. M., Howley P. M. Philadelphia: Lippincott Williams & Wilkins;
     [Google Scholar]
  26. Mayo M. A. 2002; Virology division news: ICTV at the Paris ICV: Results of the Plenary Session and the Binomial Ballot. Arch Virol 147:2254–2260
     [Google Scholar]
  27. McNulty M. S., Curran W. L., McFerran J. B. 1980; Detection of astroviruses in turkey faeces by direct electron microscopy. Vet Rec 106:561
     [Google Scholar]
  28. Mendez E., Fernandez-Luna T., Lopez S., Mendez-Toss M., Arias C. F. 2002; Proteolytic processing of a serotype 8 human astrovirus ORF2 polyprotein. J Virol 76:7996–8002
     [Google Scholar]
  29. Mendez-Toss M., Romero-Guido P., Munguia M. E., Mendez E., Arias C. F. 2000; Molecular analysis of a serotype 8 human astrovirus genome. J Gen Virol 81:2891–2897
     [Google Scholar]
  30. Monroe S. S., Jiang B., Stine S. E., Koopmans M., Glass R. I. 1993; Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses. J Virol 67:3611–3614
     [Google Scholar]
  31. Monroe S. S., Carter M. J., Herrmann J. E., Kurtz J. B., Matsui S. M. 1995; Family Astroviridae . In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses pp  364–367 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Vienna & New York: Springer-Verlag;
     [Google Scholar]
  32. Oh D., Schreier E. 2001; Molecular characterization of human astroviruses in Germany. Arch Virol 146:443–455
     [Google Scholar]
  33. Shimizu M., Shirai J., Narita M., Yamane T. 1990; Cytopathic astrovirus isolated from porcine acute gastroenteritis in an established cell line derived from porcine embryonic kidney. J Clin Microbiol 28:201–206
     [Google Scholar]
  34. Smiley J. R., Chang K. O., Hayes J., Vinje J., Saif L. J. 2002; Characterization of an enteropathogenic bovine calicivirus representing a potentially new calicivirus genus. J Virol 76:10089–10098
     [Google Scholar]
  35. Snodgrass D. R., Gray E. W. 1977; Detection and transmission of 30 nm virus particles (astroviruses) in faeces of lambs with diarrhoea. Arch Virol 55:287–291
     [Google Scholar]
  36. Snodgrass D. R., Angus K. W., Gray E. W., Menzies J. D., Paul G. 1979; Pathogenesis of diarrhoea caused by astrovirus infections in lambs. Arch Virol 60:217–226
     [Google Scholar]
  37. Todd D., Weston J. H., Soike D., Smyth J. A. 2001; Genome sequence determinations and analyses of novel circoviruses from goose and pigeon. Virology 286:354–362
     [Google Scholar]
  38. Tzipori S., Menzies J. D., Gray E. W. 1981; Detection of astrovirus in the faeces of red deer. Vet Rec 108:286
     [Google Scholar]
  39. Willcocks M. M., Brown T. D., Madeley C. R., Carter M. J. 1994; The complete sequence of a human astrovirus. J Gen Virol 75:1785–1788
     [Google Scholar]
  40. Willcocks M. M., Boxall A. S., Carter M. J. 1999; Processing and intracellular location of human astrovirus non-structural proteins. J Gen Virol 80:2607–2611
     [Google Scholar]
  41. Williams F. P. Jr 1980; Astrovirus-like, coronavirus-like, and parvovirus-like particles detected in the diarrheal stools of beagle pups. Arch Virol 66:215–226
     [Google Scholar]
  42. Woode G. N., Bridger J. C. 1978; Isolation of small viruses resembling astroviruses and caliciviruses from acute enteritis of calves. J Med Microbiol 11:441–452
     [Google Scholar]
  43. Yu M., Tang Y., Guo M., Zhang Q., Saif Y. M. 2000; Characterization of a small round virus associated with the poult enteritis and mortality syndrome. Avian Dis 44:600–610
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19267-0
Loading
Molecular characterization of a novel astrovirus associated with disease in mink
J. Gen. Virol. 84, 3087 (2003); https://doi.org/10.1099/vir.0.19267-0
/content/journal/jgv/10.1099/vir.0.19267-0
/content/journal/jgv/10.1099/vir.0.19267-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error