RT Journal Article SR Electronic(1) A1 Newcombe, Nicole G. A1 Andersson, Per A1 Johansson, E. Susanne A1 Au, Gough G. A1 Lindberg, A. Michael A1 Barry, Richard D. A1 Shafren, Darren R.YR 2003 T1 Cellular receptor interactions of C-cluster human group A coxsackieviruses JF Journal of General Virology, VO 84 IS 11 SP 3041 OP 3050 DO https://doi.org/10.1099/vir.0.19329-0 PB Microbiology Society, SN 1465-2099, AB The cellular receptor complex of coxsackievirus A21 (CVA21), a C-cluster human enterovirus, is formed by the subtle interaction of individual cellular receptors, decay accelerating factor (DAF) and intercellular adhesion molecule-1 (ICAM-1). In this receptor complex, DAF functions in the membrane sequestration of the virus, while the role of ICAM-1 is as the functional cellular internalization receptor. However, despite the elucidation of the CVA21–cell receptor interactions, there have been few definite investigations into cellular receptor usage of other coxsackie A viruses (CVAs) belonging to the C-cluster. In the present study, radiolabelled virus-binding assays demonstrated that CVA13, -15, -18 and -20, a subset of the human enterovirus C-cluster, bind directly to surface-expressed ICAM-1, but not to surface-expressed DAF. Furthermore, lytic infection of ICAM-1-expressing rhabdomyosarcoma (RD) cells by this C-cluster subset of viruses was inhibited by specific ICAM-1 monoclonal antibody blockade, except for that of CVA20. Despite possessing ICAM-1-binding capabilities, CVA20 employed an as yet unidentified internalization receptor for cell entry and subsequent productive lytic infection of ICAM-1-negative RD cells. In a further example of C-cluster cellular receptor heterogeneity, CVA13 exhibited significant binding to the surface of CHO cells expressing neither DAF nor ICAM-1. Despite a common receptor usage of ICAM-1 by this subset of C-cluster CVAs, the amino acid residues postulated to represent the ICAM-1-receptor footprint were not conserved., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19329-0