Sodium hydroxide renders the prion protein PrPSc sensitive to proteinase K

References

1. Brown P., Rohwer R. G., Gajdusek D. C. 1984; Sodium hydroxide decontamination of Creutzfeldt–Jakob disease virus. N Engl J Med 310:727
   [Google Scholar]
2. Brown P., Rohwer R. G., Gajdusek D. C. 1986; Newer data on the inactivation of scrapie virus or Creutzfeldt–Jakob disease virus in brain tissue. J Infect Dis 153:1145–1148
   [Google Scholar]
3. Caughey B., Raymond G. J., Kocisko D. A., Lansbury P. T. Jr 1997; Scrapie infectivity correlates with converting activity, protease resistance, and aggregation of scrapie-associated prion protein in guanidine denaturation studies. J Virol 71:4107–4110
   [Google Scholar]
4. Di Martino A., Safar J., Ceroni M., Gibbs C. J. Jr 1993; Inactivation of the scrapie agent in a scaled-down procedure for the purification of gangliosides from brain tissue. Dev Biol Stand 80:187–194
   [Google Scholar]
5. Flechsig E., Hegyi I., Enari M., Schwarz P., Collinge J., Weissmann C. 2001; Transmission of scrapie by steel-surface-bound prions. Mol Med 7:679–684
   [Google Scholar]
6. Foster P. R., Welch A. G., McLean C., Griffin B. D., Hardy J. C., Bartley A., MacDonald S., Bailey A. C. 2000; Studies on the removal of abnormal prion protein by processes used in the manufacture of human plasma products. Vox Sang 78:86–95
   [Google Scholar]
7. Lee D. C., Stenland C. J., Hartwell R. C. 7 other authors 2000; Monitoring plasma processing steps with a sensitive Western blot assay for the detection of the prion protein. J Virol Methods 84:77–89
   [Google Scholar]
8. Lee D. C., Stenland C. J., Miller J. L. 8 other authors 2001; A direct relationship between the partitioning of the pathogenic prion protein and transmissible spongiform encephalopathy infectivity during the purification of plasma proteins. Transfusion 41:449–455 erratum 41, 1079
   [Google Scholar]
9. McKinley M. P., Bolton D. C., Prusiner S. B. 1983; A protease-resistant protein is a structural component of the scrapie prion. Cell 35:57–62
   [Google Scholar]
10. Meyer R. K., McKinley M. P., Bowman K. A., Braunfeld M. B., Barry R. A., Prusiner S. B. 1986; Separation and properties of cellular and scrapie prion proteins. Proc Natl Acad Sci U S A 83:2310–2314
    [Google Scholar]
11. Miller J. L., Petteway S. R. Jr, Lee D. C. 2001; Ensuring the pathogen safety of intravenous immunoglobulin and other human plasma-derived therapeutic proteins. J Allergy Clin Immunol 108 (Suppl. 4):S91–S94
    [Google Scholar]
12. Oesch B., Doherr M., Heim D. 7 other authors 2000; Application of Prionics Western blotting procedure to screen for BSE in cattle regularly slaughtered at Swiss abattoirs. Arch Virol 16:189–195
    [Google Scholar]
13. Prusiner S. B. 1998; Prions. Proc Natl Acad Sci U S A 95:13363–13383
    [Google Scholar]
14. Prusiner S. B., Groth D. F., McKinley M. P., Cochran S. P., Bowman K. A., Kasper K. C. 1981; Thiocyanate and hydroxyl ions inactivate the scrapie agent. Proc Natl Acad Sci U S A 78:4606–4610
    [Google Scholar]
15. Race R., Jenny A., Sutton D. 1998; Scrapie infectivity and proteinase K-resistant prion protein in sheep placenta, brain, spleen, and lymph node: implications for transmission and antemortem diagnosis. J Infect Dis 178:949–953
    [Google Scholar]
16. Safar J., Wang W., Padgett M. P., Ceroni M., Piccardo P., Zopf D., Gajdusek D. C., Gibbs C. J. Jr 1990; Molecular mass, biochemical composition, and physicochemical behavior of the infectious form of the scrapie precursor protein monomer. Proc Natl Acad Sci U S A 87:6373–6377
    [Google Scholar]
17. Safar J., Roller P. P., Gajdusek D. C., Gibbs C. J. Jr 1993; Conformational transitions, dissociation, and unfolding of scrapie amyloid (prion) protein. J Biol Chem 268:20276–20284
    [Google Scholar]
18. Schaller O., Fatzer R., Stack M. 9 other authors 1999; Validation of a Western immunoblotting procedure for bovine PrP^Sc detection and its use as a rapid surveillance method for the diagnosis of bovine spongiform encephalopathy (BSE). Acta Neuropathol 98:437–443
    [Google Scholar]
19. Shaked G. M., Fridlander G., Meiner Z., Taraboulos A., Gabizon R. 1999; Protease-resistant and detergent-insoluble prion protein is not necessarily associated with prion infectivity. J Biol Chem 274:17981–17986
    [Google Scholar]
20. Somerville R. A. 2002; TSE agent strains and PrP: reconciling structure and function. Trends Biochem Sci 27:606–612
    [Google Scholar]
21. Somerville R. A., Oberthur R. C., Havekost U., MacDonald F., Taylor D. M., Dickinson A. G. 2002; Characterization of thermodynamic diversity between transmissible spongiform encephalopathy agent strains and its theoretical implications. J Biol Chem 277:11084–11089
    [Google Scholar]
22. Tateishi J., Tashima T., Kitamoto T. 1991; Practical methods for chemical inactivation of Creutzfeldt–Jakob disease pathogen. Microbiol Immunol 35:163–166
    [Google Scholar]
23. Taylor D. M. 2000; Inactivation of transmissible degenerative encephalopathy agents. Vet J 159:10–17
    [Google Scholar]
24. Wille H., Prusiner S. B., Cohen F. E. 2000; Scrapie infectivity is independent of amyloid staining properties of the N-terminally truncated prion protein. J Struct Biol 130:323–338
    [Google Scholar]