1887

Abstract

To evaluate the potential of chemically synthesized lipopeptides for vaccination against foot-and-mouth disease (FMD), seven lipopeptides containing the immunostimulating principle of bacterial lipoproteins and linear B-cell epitopes of FMDV strain OKaufbeuren (OK) were used to immunize cattle (=7). Animals were vaccinated once and 21 days after immunization animals were infected with the homologous virus. Four animals were protected. After vaccination, as well as after challenge infection, B- and T-cell responses were examined. Sera were tested for virus- and peptide-specific antibodies and showed after vaccination only a weak antibody response. After challenge infection, an increase in antibody titre was obvious but there was no correlation between antibody titre and protection. The reactivity of the cellular immune system was detected by analyses of PBMCs for virus- and peptide-specific T-lymphocytes. With regard to the virus-specific T-lymphocytes, a heterogeneous reactivity could be shown. No correlation between virus-specific T-cell proliferation and protection was found. Obvious was the fact that all protected animals showed after vaccination a strong T-cell response against at least one of the peptides used for immunization. These results suggest a correlation between the onset of an antigen-specific T-cell reaction and protection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19366-0
2003-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/84/12/vir843315.html?itemId=/content/journal/jgv/10.1099/vir.0.19366-0&mimeType=html&fmt=ahah

References

  1. Acharya R., Fry E., Stuart D., Fox G., Rowlands D. J., Brown F. 1989; The three-dimensional structure of foot-and-mouth disease virus at 2·9 Å resolution. Nature 337:709–716
    [Google Scholar]
  2. Aliprantis A. O., Yang R. B., Mark M. R., Suggett S., Devaux B., Radolf J. D., Klimpel G. R., Godowski P., Zychlinsky A. 1999; Cell activation and apoptosis by bacterial lipoproteins through Toll-like receptor-2. Science 285:736–739
    [Google Scholar]
  3. Barteling S. J., Vreeswijk J. 1991; Developments in foot-and-mouth disease vaccines. Vaccine 9:75–88
    [Google Scholar]
  4. Bittle J. L., Houghten R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J., Brown F. 1982; Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature 298:30–33
    [Google Scholar]
  5. Brown F. 1992; New approaches to vaccination against foot-and-mouth disease. Vaccine 10:1022–1026
    [Google Scholar]
  6. Collen T. 1994; Foot-and-mouth disease virus (aphthovirus): viral T cell epitopes. In Cell-Mediated Immunity in Ruminants pp  173–197 Edited by Goddeeris B. M. I., Morrison I. Boca Raton: CRC Press;
    [Google Scholar]
  7. Collen T., DiMarchi R., Doel T. R. 1991; A T cell epitope in VP1 of foot-and-mouth disease virus is immunodominant for vaccinated cattle. J Immunol 146:749–755
    [Google Scholar]
  8. De Mattia F., Chomez S., Van Laethem F., Moulin V., Urbain J., Moser M., Leo O., Andris F. 1999; Antigen-experienced T cells undergo a transient phase of unresponsiveness following optimal stimulation. J Immunol 163:5929–5936
    [Google Scholar]
  9. DiMarchi R., Brooke G., Gale C., Cracknell V., Doel T., Mowat N. 1986; Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science 232:639–641
    [Google Scholar]
  10. Doel T. R., Gale C., Do Amaral C. M. C. F., Mulcahy G., DiMarchi R. 1990; Heterotypic protection induced by synthetic peptides corresponding to three serotypes of foot-and-mouth disease virus. J Virol 64:2260–2264
    [Google Scholar]
  11. Domingo E., Mateu M. G., Martínez M. A., Dopazo J., Moya A., Sobrino F. 1990 Applied Virology Research , vol. 2 pp  233–266 Edited by Kurstak E., Marusyk R. G., Murphy B. New York: Plenum;
    [Google Scholar]
  12. Forss S., Strebel K., Beck E., Schaller H. 1984; Nucleotide sequence and genome organization of foot-and-mouth disease virus. Nucleic Acids Res 12:6587–6601
    [Google Scholar]
  13. Hamblin C., Barnett I. T., Hedger R. S. 1986; A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. I. Development and method of ELISA. J Immunol Methods 93:115–121
    [Google Scholar]
  14. McCullough K. C., Bruckner L., Schaffner R., Fraefel W., Müller H. K., Kihm U. 1992; Relationship between the anti-FMD virus antibody reaction as measured by different assays, and protection in vivo against challenge infection. Vet Microbiol 30:99–112
    [Google Scholar]
  15. Merrifield R. B., Stewart J. M. 1965; Automated peptide synthesis. Nature 207:522–523
    [Google Scholar]
  16. Morgan D. O., Moore D. M. 1990; Protection of cattle and swine against foot-and-mouth disease, using biosynthetic peptide vaccines. Am J Vet Res 51:40–45
    [Google Scholar]
  17. Pereira H. G. 1981; Virus Diseases of Food Animals . pp  333–363 Edited by Gibbs E. P. London, UK: Academic Press;
  18. Pfaff E., Mussgay M., Böhm H. O., Schulz G. E., Schaller H. 1982; Antibodies against a preselected peptide recognize and neutralize foot-and-mouth disease virus. EMBO J 1:869–874
    [Google Scholar]
  19. Riley E. M. 1996; The role of MHC- and non-MHC-associated genes in determining the human immune response to malaria antigens. Parasitology 112:39–51
    [Google Scholar]
  20. Sobrino F., Blanco E., Garcia-Briones M., Ley V. 1999; Synthetic peptide vaccines: foot-and-mouth disease virus as a model. Dev Biol Stand 101:39–43
    [Google Scholar]
  21. Strohmaier K., Franze R., Adam K. H. 1982; Location and characterization of the antigenic portion of the FMDV immunizing protein. J Gen Virol 59:295–306
    [Google Scholar]
  22. Taboga O., Tami C., Carrello E. 13 other authors 1997; A large-scale evaluation of peptide vaccines against foot-and-mouth disease: lack of solid protection in cattle and isolation of escape mutants. J Virol 71:2606–2614
    [Google Scholar]
  23. Volpina O. M., Surovoy A. Y., Zhmak M. N., Kuprianova M. A., Koroev D. O., Chepurkin A. V., Toloknov A. S., Ivanov V. T. 1999; A peptide construct containing B-cell and T-cell epitopes from the foot-and-mouth disease viral VP1 protein induces efficient antiviral protection. Vaccine 17:577–584
    [Google Scholar]
  24. Wiesmüller K.-H., Jung G., Hess G. 1989; Novel low-molecular-weight synthetic vaccine against foot-and-mouth disease containing a potent B-cell and macrophage activator. Vaccine 7:29–33
    [Google Scholar]
  25. Wiesmüller K.-H., Bessler W. G., Jung G. 1992; Solid phase peptide synthesis of lipopeptide vaccines eliciting epitope-specific B-, T-helper and T-killer cell response. Int J Pept Protein Res 40:255–260
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19366-0
Loading
/content/journal/jgv/10.1099/vir.0.19366-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error