RT Journal Article SR Electronic(1) A1 Borzacchiello, Giuseppe A1 Iovane, Giuseppe A1 Marcante, Maria Luisa A1 Poggiali, Federica A1 Roperto, Franco A1 Roperto, Sante A1 Venuti, AldoYR 2003 T1 Presence of bovine papillomavirus type 2 DNA and expression of the viral oncoprotein E5 in naturally occurring urinary bladder tumours in cows JF Journal of General Virology, VO 84 IS 11 SP 2921 OP 2926 DO https://doi.org/10.1099/vir.0.19412-0 PB Microbiology Society, SN 1465-2099, AB Samples of neoplastic and normal urothelium were obtained from cows originating from areas of southern Italy, a region in which chronic enzootic haematuria is endemic and bracken fern infestation is widespread. Specimens were analysed for bovine papillomavirus type 2 (BPV-2) DNA, BPV-2 E5 expression and telomerase activity. A total of 46 of 60 tumours and 17 of 34 normal bladder mucosa samples harboured BPV-2 DNA. Analysis of a subset of samples showed E5 protein expression and telomerase activity in tumour tissue only. No normal samples positive for BPV DNA showed E5 protein expression or telomerase activity, suggesting the presence of DNA in a latent state. Taken together, these data on naturally occurring bovine bladder tumours corroborate the hypothesis of their virus origin., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19412-0