1887

Abstract

The putative G glycoprotein genes of 25 human metapneumovirus (hMPV) field isolates obtained during five consecutive epidemic seasons (1997 to 2002) were sequenced. Sequence alignments identified two major genetic groups, designated groups 1 and 2, and two minor genetic clusters within each major group, designated subgroups A and B. Extensive nucleotide and deduced amino acid sequence variability was observed, consisting of high rates of nucleotide substitutions, use of alternative transcription-termination codons and insertions that retained the reading frame. Deduced amino acid sequences showed the greatest variability, with most differences located in the extracellular domain of the protein: nucleotide and amino acid sequence identities for the entire open reading frame ranged from 52 to 58 % and 31 to 35 %, respectively, between the two major groups. Like the closely related avian pneumovirus and human and bovine respiratory syncytial viruses, the predicted G protein of hMPV shared the basic features of a type II mucin-like glycosylated protein. However, differences from these related viruses were also observed, e.g. lack of conserved cysteine clusters as seen in human respiratory syncytial virus and avian pneumovirus. The displacement of genetic groups of hMPV observed during the study period suggests that potential antigenic differences in the G glycoprotein, which have evolved in response to immune-mediated pressure, may influence the circulation patterns of hMPV strains.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19504-0
2004-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/3/vir850679.html?itemId=/content/journal/jgv/10.1099/vir.0.19504-0&mimeType=html&fmt=ahah

References

  1. Alvarez R., Lwamba H. M., Kapczynski D. R., Njenga M. K., Seal B. S. 2003; Nucleotide and predicted amino acid sequence-based analysis of the avian metapneumovirus type C cell attachment glycoprotein gene: phylogenetic analysis and molecular epidemiology of US pneumoviruses. J Clin Microbiol 41:1730–1735
    [Google Scholar]
  2. Bastien N., Normand S., Taylor T., Ward D., Peret T. C. T., Boivin G., Anderson L. J., Li Y. 2003; Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains. Virus Res 9351–62
    [Google Scholar]
  3. Bäyon-Auboyer M.-H., Arnauld C., Toquin D., Eterradossi N. 2000; Nucleotide sequences of the F, L and G protein genes of two non-A/non-B avian pneumoviruses (APV) reveal a novel APV subgroup. J Gen Virol 81:2723–2733
    [Google Scholar]
  4. Biacchesi S., Skiadopoulos M. H., Boivin G., Hanson C. T., Murphy B. R., Collins P. L., Buchholz U. J. 2003; Genetic diversity between human metapneumovirus subgroups. Virology 315:1–9
    [Google Scholar]
  5. Boivin G., Abed Y., Pelletier G. 6 other authors 2002; Virological features and clinical manifestations associated with human metapneumovirus: a new paramyxovirus responsible for acute respiratory-tract infections in all age groups. J Infect Dis 186:1330–1334
    [Google Scholar]
  6. Boivin G., De Serres G., Cote S., Gilca R., Abed Y., Rochette L., Bergeron M. G., Dery P. 2003; Human metapneumovirus infections in hospitalized children. Emerg Infect Dis 9:634–640
    [Google Scholar]
  7. Cane P. A., Pringle C. R. 1995; Evolution of subgroup A respiratory syncytial virus: evidence for progressive accumulation of amino changes in the attachment protein. J Virol 69:2918–2925
    [Google Scholar]
  8. Cane P. A., Matthews D. A., Pringle C. R. 1994; Analysis of respiratory syncytial virus strain variation in successive epidemics in one city. J Clin Microbiol 32:1–4
    [Google Scholar]
  9. Elena S. F., Miralles R., Cuevas J. M., Turner P. E., Moya A. 2000; The two faces of mutation: extinction and adaptation in RNA viruses. IUBMB Life 49:5–9
    [Google Scholar]
  10. Elvander M., Vilcek S., Baule C., Uttenthal A., Ballagi-Pordany A., Belak S. 1998; Genetic and antigenic analysis of the G attachment protein of bovine respiratory syncytial virus strains. J Gen Virol 79:2939–2946
    [Google Scholar]
  11. Falsey A. R., Erdman D. D., Anderson L. J., Walsh E. E. 2003; Human metapneumovirus infections in young and elderly adults. J Infect Dis 187:785–790
    [Google Scholar]
  12. Furze J. M., Roberts S. R., Wertz G. W., Taylor G. 1997; Antigenically distinct G glycoproteins of BRSV strains share a high degree of genetic homogeneity. Virology 231:48–58
    [Google Scholar]
  13. Jartti T., van den Hoogen B., Garofalo R. P., Osterhaus A. D., Ruuskanen O. 2002; Metapneumovirus and acute wheezing in children. Lancet 360:1393–1394
    [Google Scholar]
  14. Johnson P. R., Spriggs M. K., Olmsted R. A., Collins P. L. 1987; The G glycoprotein of human respiratory syncytial viruses of subgroups A and B: extensive sequence divergence between antigenically related proteins. Proc Natl Acad Sci U S A 84:5625–5629
    [Google Scholar]
  15. Juhasz K., Easton A. J. 1994; Extensive sequence variation in the attachment (G) protein gene of avian pneumovirus: evidence for two distinct subgroups. J Gen Virol 75:2873–2880
    [Google Scholar]
  16. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. J Mol Biol 157:105–132
    [Google Scholar]
  17. Lapthorn A. J., Janes R. W., Isaacs N. W., Wallace B. A. 1995; Cystine nooses and protein specificity. Nat Struct Biol 2:266–268
    [Google Scholar]
  18. Larsen L. E., Uttenthal A., Arctander P. 6 other authors 1998; Serological and genetic characterization of bovine respiratory syncytial virus (BRSV) indicates that Danish isolates belong to the intermediate subgroup: no evidence of a selective effect on the variability of G protein nucleotide sequence by prior cell culture adaptation and passages in cell culture or calves. Vet Microbiol 62:265–279
    [Google Scholar]
  19. Ling R., Easton A. J., Pringle C. R. 1992; Sequence analysis of the 22K, SH and G genes of turkey rhinotracheitis virus and their intergenic regions reveals a gene order different from that of other pneumoviruses. J Gen Virol 73:1709–1715
    [Google Scholar]
  20. Martinez I., Valdes O., Delfraro A., Arbiza J., Russi J., Melero J. A. 1999; Evolutionary pattern of the G glycoprotein of human respiratory syncytial viruses from antigenic group B: the use of alternative termination codons and lineage diversification. J Gen Virol 180:125–130
    [Google Scholar]
  21. Pelletier G., Dery P., Abed Y., Boivin G. 2002; Respiratory tract reinfections by the new human metapneumovirus in an immunocompromised child. Emerg Infect Dis 8:976–978
    [Google Scholar]
  22. Peret T. C. T., Hall C. B., Schnabel K. C., Golub J. A., Anderson L. J. 1998; Circulation patterns of genetically distinct group A and B strains of human respiratory syncytial virus in a community. J Gen Virol 79:2221–2229
    [Google Scholar]
  23. Peret T. C. T., Boivin G., Li Y., Couillard M., Humphrey C., Osterhaus A. D., Erdman D. D., Anderson L. J. 2002; Characterization of human metapneumoviruses isolated from patients in North America. J Infect Dis 185:1660–1663
    [Google Scholar]
  24. Schlender J., Zimmer G., Herrler G., Conzelmann K. K. 2003; Respiratory syncytial virus (RSV) fusion protein subunit F2, not attachment protein G, determines the specificity of RSV infection. J Virol 77:4609–4616
    [Google Scholar]
  25. Sparer T. E., Matthews S., Hussell T., Rae A. J., Garcia-Barreno B., Melero J. A., Openshaw P. J. 1998; Eliminating a region of respiratory syncytial virus attachment protein allows induction of protective immunity without vaccine-enhanced lung eosinophilia. J Exp Med 187:1921–1926
    [Google Scholar]
  26. Stockton J., Stephenson I., Fleming D., Zambon M. 2002; Human metapneumovirus as a cause of community-acquired respiratory illness. Emerg Infect Dis 8:897–901
    [Google Scholar]
  27. Sullender W. M., Mufson M. A., Anderson L. J., Wertz G. W. 1991; Genetic diversity of the attachment protein of subgroup B respiratory syncytial viruses. J Virol 65:5425–5434
    [Google Scholar]
  28. Swofford D. L. 1999 PAUP*. Phylogenetic Analysis using Parsimony (* and Other Methods) Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  29. Teng M. N., Collins P. L. 2002; The central conserved cystine noose of the attachment G protein of human respiratory syncytial virus is not required for efficient viral infection in vitro or in vivo. J Virol 76:6164–6171
    [Google Scholar]
  30. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  31. Tripp R. A., Jones L. P., Haynes L. M., Zheng H., Murphy P. M., Anderson L. J. 2001; CX3C chemokine mimicry by respiratory syncytial virus G glycoprotein. Nat Immunol 2:732–738
    [Google Scholar]
  32. Valarcher J. F., Schelcher F., Bourhy H. 2000; Evolution of bovine respiratory syncytial virus. J Virol 74:10714–10728
    [Google Scholar]
  33. van den Hoogen B. G., de Jong J. C., Groen J., Kuiken T., de Groot R., Fouchier R. A. M., Osterhaus A. D. M. E. 2001; A newly discovered human pneumovirus isolated from young children with respiratory tract disease. Nat Med 7:719–724
    [Google Scholar]
  34. van den Hoogen B. G., Bestebroer T. M., Osterhaus A. D. M. E., Fouchier R. A. M. 2002; Analysis of the genomic sequence of a human metapneumovirus. Virology 295:119–132
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19504-0
Loading
/content/journal/jgv/10.1099/vir.0.19504-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error