1887

Abstract

Equine arteritis virus (EAV) causes a persistent infection of the reproductive tract of carrier stallions. The authors determined the complete genome sequences of viruses (CW96 and CW01) that were present 5 years apart in the semen of a carrier stallion (CW). The CW96 and CW01 viruses respectively had only 85·6 % and 85·7 % nucleotide identity to the published sequence of EAV (EAV030). The CW96 and CW01 viruses had two 1 nt insertions and a single 1 nt deletion in the leader sequence, and a 3 nt coding insertion in ORF1a; thus their genomes included 12 708 nt as compared to the 12 704 nt in EAV030. Variation between viruses present in the semen of stallion CW and EAV030 was especially marked in the replicase gene (ORF1a and 1b), and the greatest variation occurred in the portion of ORF1a encoding the nsp2 protein. The ORFs 3 and 5, which respectively encode the GP3 and GP5 envelope proteins, showed greatest variation amongst ORFs encoding structural EAV proteins. Comparative sequence analyses of CW96 and CW01 indicated that ORFs 1a, 1b and 7 were highly conserved during persistent infection, whereas there was substantial variation in ORFs 3 and 5. Although the variation that occurs in ORF5 results in the emergence of novel phenotypic viral variants as determined by neutralization assay, all variants were neutralized by high-titre polyclonal equine antisera, suggesting that immune evasion is unlikely to be responsible for the establishment of persistent EAV infection of carrier stallions. Northern blot analyses of RNA extracted from cell culture propagated viruses isolated from 10 different persistently infected stallions failed to demonstrate any large genomic deletions, suggesting that defective interfering particles are also unlikely to be important in either the maintenance or clearance of persistent EAV infection of the reproductive tract of carrier stallions.

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2004-02-01
2024-03-29
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References

  1. Balasuriya U. B. R., Rossitto P. V., DeMaula C. D., MacLachlan N. J. 1993; A 29K envelope glycoprotein of equine arteritis virus expresses neutralization determinants recognized by murine monoclonal antibodies. J Gen Virol 74:2525–2529
    [Google Scholar]
  2. Balasuriya U. B. R., MacLachlan N. J., de Vries A. A. F., Rossitto P. V., Rottier P. J. M. 1995a; Identification of a neutralization site in the major envelope glycoprotein (GL) of equine arteritis virus. Virology 207:518–527
    [Google Scholar]
  3. Balasuriya U. B. R., Timoney P. J., McCollum W. H., MacLachlan N. J. 1995b; Phylogenetic analysis of open reading frame 5 of field isolates of equine arteritis virus and identification of conserved and nonconserved regions in the GL envelope glycoprotein. Virology 214:690–697
    [Google Scholar]
  4. Balasuriya U. B. R., Patton J. F., Rossitto P. V., Timoney P. J., McCollum W. H., MacLachlan N. J. 1997; Neutralization determinants of laboratory strains and field isolates of equine arteritis virus: identification of four neutralization sites in the amino-terminal ectodomain of the GL envelope glycoprotein. Virology 232:114–128
    [Google Scholar]
  5. Balasuriya U. B. R., Evermann J. F., Hedges J. F., McKeirnan A. J., Mitten J. Q., Beyer J. C., McCollum W. H., Timoney P. J., MacLachlan N. J. 1998; Serologic and molecular characterization of an abortigenic strain of equine arteritis virus derived from infective frozen semen and an aborted equine fetus. J Am Vet Med Assoc 213:1586–1589
    [Google Scholar]
  6. Balasuriya U. B. R., Hedges J. F., Timoney P. J., McCollum W. H., MacLachlan N. J. 1999; Genetic stability of equine arteritis virus during horizontal and vertical transmission in an outbreak of equine viral arteritis. J Gen Virol 80:1949–1958
    [Google Scholar]
  7. Balasuriya U. B. R., Heidner H. W., Hedges J. F., Williams J. C., Davis N. L., Johnston R. E., MacLachlan N. J. 2000; Expression of two major envelope proteins of equine arteritis virus as a heterodimer is necessary for induction of neutralizing antibodies in mice immunized with recombinant Venezuelan equine encephalitis virus replicon particles. J Virol 74:10623–10630
    [Google Scholar]
  8. Balasuriya U. B. R., Hedges J. F., MacLachlan N. J. 2001; Molecular epidemiology and evolution of equine arteritis virus. Adv Exp Med Biol 494:19–24
    [Google Scholar]
  9. Balasuriya U. B. R., Heidner H. W., Davis N. L. 7 other authors 2002; Alphavirus replicon particles expressing the two major envelope proteins of equine arteritis virus induce high level protection against challenge with virulent virus in vaccinated horses. Vaccine 20:1609–1627
    [Google Scholar]
  10. Cavanagh D. 1997; Nidovirales: a new order comprising coronaviridae and arteriviridae. Arch Virol 142:629–633
    [Google Scholar]
  11. Cowley J. A., Walker P. J. 2002; The complete sequence of gill-associated virus of Penacus monodon prawns indicates a gene organization unique amongst nidoviruses. Arch Virol 147:1977–1987
    [Google Scholar]
  12. den Boon J. A., Snijder E. J., Chirnside E. D., de Vries A. A. F., Horzinek M. C., Spaan W. J. M. 1991; Equine arteritis virus is not a togavirus but belongs to the coronaviruslike superfamily. J Virol 65:2910–2920
    [Google Scholar]
  13. Deregt D., de Vries A. A. F., Raamsman M. J. B., Elmgren L. D., Rottier P. J. M. 1994; Monoclonal antibodies to equine arteritis virus proteins identify the GL protein as a target for virus neutralization. J Gen Virol 75:2439–2444
    [Google Scholar]
  14. de Vries A. A. F., Chirnside E. D., Horzinek M. C., Rottier P. J. M. 1992; Structural proteins of equine arteritis virus. J Virol 66:6294–6303
    [Google Scholar]
  15. de Vries A. A. F., Post S. M., Raamsman M. J. B., Horzinek M. C., Rottier P. J. M. 1995; The two major envelope proteins of equine arteritis virus associate into disulfide-linked heterodimers. J Virol 69:4668–4674
    [Google Scholar]
  16. de Vries A. A. F., Horzinek M. C., Rottier P. J. M., de Groot R. J. 1997; The genome organization of the nidovirales: similarities and differences between arteri-, toro- and coronaviruses. Semin Virol 8:33–47
    [Google Scholar]
  17. Doll E. R., Bryans J. T., McCollum W. H., Crowe M. E. W. 1957; Isolation of a filterable agent causing arteritis of horses and abortion by mares. Its differentiation from the equine abortion (influenza) virus. Cornell Vet 47:3–41
    [Google Scholar]
  18. Drolet B. S., Chiou P. P., Heidel J., Leong J. C. 1995; Detection of truncated virus particles in a persistent RNA virus infection in vivo . J Virol 69:2140–2147
    [Google Scholar]
  19. Felsenstein J. 1993; PHYLIP (Phylogeny Inference Package) 3.5c Manual. Department of Genetics SK-50, University of Washington; Seattle, WA:
  20. Frank S. A. 2000; Within-host spatial dynamics of viruses and defective interfering particles. J Theor Biol 206:279–290
    [Google Scholar]
  21. Glaser A. L., de Vries A. A. F., Dubovi E. J. 1995; Comparison of equine arteritis virus isolates using neutralizing monoclonal antibodies and identification of sequence changes in GL associated with neutralization resistance. J Gen Virol 76:2223–2233
    [Google Scholar]
  22. Glaser A. L., Rottier P. J. M., Horzinek M. C., Colenbrander B. 1996; Equine arteritis virus: a review of clinical features and management aspects. Vet Q 18:95–99
    [Google Scholar]
  23. Glaser A. L., Chirnside E. D., Horzinek M. C., de Vries A. A. F. 1997; Equine arteritis virus. Theriogenology 47:1275–1295
    [Google Scholar]
  24. Hedges J. F., Balasuriya U. B. R., Timoney P. J., McCollum W. H., MacLachlan N. J. 1999; Genetic divergence with emergence of phenotypic variants of equine arteritis virus during persistent infection of stallions. J Virol 73:3672–3681
    [Google Scholar]
  25. Hedges J. F., Balasuriya U. B. R., Topol J., MacLachlan N. J. 2001; Genetic variation of ORFs 3 and 4 of equine arteritis virus. Adv Exp Med Biol 494:69–72
    [Google Scholar]
  26. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Science 226:325–327
    [Google Scholar]
  27. Huang A. S., Baltimore D. 1977; Defective interfering animal viruses. In Comprehensive Virology pp  73–116 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum;
    [Google Scholar]
  28. Jitrapakdee S., Unajak S., Sittidilokranta N., Hodgson R. A. J., Cowley J. A., Walker P. J., Panyim S., Boonsaeng V. 2003; Identification and analysis of gp116 and gp64 structural glycoproteins of yellow head nidovirus of Penaeus monodon shrimp. J Gen Virol 84:863–873
    [Google Scholar]
  29. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001 MEGA2.0: Molecular Evolutionary Genetics Analysis software Tempe, AZ: Arizona State University;
    [Google Scholar]
  30. Larsen L. E., Storgaard T., Holmback K. 2001; Phylogenetic characterization of the GL sequence of equine arteritis virus isolated from semen of asymptomatic stallions and fatal cases of equine viral arteritis. Vet Microbiol 80:339–346
    [Google Scholar]
  31. Li W.-H., Wu C.-I., Luo C.-C. 1985; A new method for estimating synonymous and nonsynonymous rates of nucleotide substitution considering the relative likelihood of nucleotide and codon changes. Mol Biol Evol 2:150–174
    [Google Scholar]
  32. McCollum W. H., Timoney P. J. 1999; Experimental observation on the virulence of isolates of equine arteritis virus. In Equine Infectious Diseases VIII pp  558–559 Edited by Wernery U., Wade J. F., Mumford J. A., Kaaden O.-R. Newmarket, UK: R & W Publications;
    [Google Scholar]
  33. McCollum W. H., Timoney P. J., Lee J. W. Jr, Habacker P. L., Balasuriya U. B. R., MacLachlan N. J. 1999; Features of an outbreak of equine viral arteritis on a breeding farm associated with abortion and fatal interstitial pneumonia in neonatal foals. In Equine Infectious Diseases VIII pp  559–560 Edited by Wernery U., Wade J. F., Mumford J. A., Kaaden O.-R. Newmarket, UK: R & W Publications;
    [Google Scholar]
  34. Molenkamp R., Rozier B. C. D., Greve S., Spaan W. J. M., Snijder E. J. 2000; Isolation and characterization of an arterivirus defective interfering RNA genome. J Virol 74:3156–3165
    [Google Scholar]
  35. Nelsen C. J., Murtaugh M. P., Faaberg K. S. 1999; Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents. J Virol 73:270–280
    [Google Scholar]
  36. Patton J. F., Balasuriya U. B. R., Hedges J. F., Schweidler T. M., Hullinger P. J., MacLachlan N. J. 1999; Phylogenetic characterization of a highly attenuated strain of equine arteritis virus from the semen of a persistently infected standardbred stallion. Arch Virol 144:817–827
    [Google Scholar]
  37. Roux L., Simon A. E., Holland J. J. 1991; Effects of defective interfering viruses on virus replication and pathogenesis in vitro and in vivo. Adv Virus Res 40:181–211
    [Google Scholar]
  38. Shen S., Kwang J., Liu W., Liu D. X. 2000; Determination of the complete nucleotide sequence of a vaccine strain of porcine reproductive and respiratory syndrome virus and identification of the Nsp2 gene with a unique insertion. Arch Virol 145:871–883
    [Google Scholar]
  39. Snijder E. J. 2001; Arteriviruses. In Fields Virology pp  1205–1220 Edited by Knipe D. M., Howley P. M. Philadelphia, PA: Lippincott Williams & Wilkins;
    [Google Scholar]
  40. Snijder E. J., Meulenberg J. J. M. 1998; The molecular biology of arteriviruses. J Gen Virol 79:961–979
    [Google Scholar]
  41. Snijder E. J., van Tol H., Pedersen K. W., Raamsman M. J. B., de Vries A. A. F. 1999; Identification of a novel structural protein of arteriviruses. J Virol 73:6335–6345
    [Google Scholar]
  42. Snijder E. J., van Tol H., Roos N., Pedersen K. W. 2001; Non-structural proteins 2 and 3 interact to modify host cell membranes during the formation of the arterivirus replication complex. J Gen Virol 82:985–994
    [Google Scholar]
  43. Snijder E. J., Dobbe J. C., Spaan W. J. M. 2003; Heterodimerization of the two major envelope proteins is essential for arterivirus infectivity. J Virol 77:97–104
    [Google Scholar]
  44. Stadejek T., Bjorklund H., Bascunana C. R. 9 other authors 1999; Genetic diversity of equine arteritis virus. J Gen Virol 80:691–699
    [Google Scholar]
  45. Tautz N., Theil H.-J., Dubovi E. J., Meyers G. 1994; Pathogenesis of mucosal disease: a cytopathogenic pestivirus generated by an internal deletion. J Virol 68:3289–3297
    [Google Scholar]
  46. Tijms M. A., van Dinten L. C., Gorbalenya A. E., Snijder E. J. 2001; A zinc finger-containing papain-like protease couples subgenomic mRNA synthesis to genome translation in positive-stranded RNA virus. Proc Natl Acad Sci U S A 98:1889–1894
    [Google Scholar]
  47. Timoney P. J. 1999; Equids and equine semen: international trade vs. disease control. In Equine Infectious Diseases VIII pp  328–331 Edited by Wernery U., Wade J. F., Mumford J. A., Kaaden O.-R. Newmarket: R & W Publications;
    [Google Scholar]
  48. Timoney P. J., McCollum W. H. 1993; Equine viral arteritis. Vet Clin North Am Equine Pract 9:295–309
    [Google Scholar]
  49. Timoney P. J., McCollum W. H., Roberts A. W., Murphy T. W. 1986; Demonstration of the carrier state in naturally acquired equine arteritis virus infection in the stallion. Res Vet Sci 41:279–280
    [Google Scholar]
  50. Timoney P. J., McCollum W. H., Murphy T. W., Roberts A. W., Willard J. G., Carswell G. D. 1987; The carrier state in equine arteritis virus infection in the stallion with specific emphasis on the venereal mode of virus transmission. J Reprod Fertil Suppl 35:95–102
    [Google Scholar]
  51. van Dinten L. C. 1999; Equine arteritis virus replicative proteins . PhD dissertation University of Leiden; The Netherlands: pp  1–113
  52. van Dinten L. C., den Boon J. A., Wassenaar A. L. M., Spaan W. J. M., Snijder E. J. 1997; An infectious arterivirus cDNA clone: identification of a replicase point mutation that abolishes discontinuous mRNA transcription. Proc Natl Acad Sci U S A 94:991–996
    [Google Scholar]
  53. Wieringa R., de Vries A. A. F., Raamsman M. J., Rottier P. J. 2003; Characterization of two new structural glycoproteins, GP(3) and GP(4), of equine arteritis virus. J Virol 76:10829–10840
    [Google Scholar]
  54. Yang Z., Nielsen R. 2000; Estimating synonymous and nonsynonymous substitution rates under realistic evolutionary models. Mol Biol Evol 17:32–43
    [Google Scholar]
  55. Ziebuhr J., Snijder E. J., Gorbalenya A. E. 2000; Virus-encoded proteinases and proteolytic processing in the nidoviruses. J Gen Virol 81:853–879
    [Google Scholar]
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