RT Journal Article SR Electronic(1) A1 Wang, Xiaochun A1 Zhang, Jiamin A1 Lu, Jie A1 Yi, Fuming A1 Liu, Chuanfeng A1 Hu, YuanyangYR 2004 T1 Sequence analysis and genomic organization of a new insect picorna-like virus, Ectropis obliqua picorna-like virus, isolated from Ectropis obliqua JF Journal of General Virology, VO 85 IS 5 SP 1145 OP 1151 DO https://doi.org/10.1099/vir.0.19638-0 PB Microbiology Society, SN 1465-2099, AB The complete nucleotide sequence of a new insect picorna-like virus, Ectropis obliqua picorna-like virus (EoPV), which causes a fatal infection of Ectropis obliqua larvae, has been determined. The genomic RNA of EoPV is 9394 nt in length and contains a single, large open reading frame (nt 391–9351) encoding a polyprotein of 2987 aa. Sequence comparisons with other viral polyproteins revealed that the consensus sequences for picornavirus RNA helicase, protease and RNA-dependent RNA polymerase proteins are found on the genome in order in the 5′→3′ direction. All structural genes were located at the 5′ terminus. In terms of sequence similarity, identity and genome organization, EoPV resembles mammalian picornaviruses and three other insect picorna-like viruses: Infectious flacherie virus of silkworm, Sacbrood virus of honeybee and Perina nuda picorna-like virus (PnPV). Phylogenetic analysis showed that EoPV is most closely related to PnPV and suggests that these four insect picorna-like viruses might constitute a new group of insect-infectious RNA viruses., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.19638-0